文献类型：期刊文献

英文题名：Host Specificity and Source of Enterocytozoon bieneusi Genotypes in a Drinking Source Watershed

作者：Guo, Yaqiong[1,2];Alderisio, Kerri A.[3];Yang, Wenli[2];Cama, Vitaliano[4];Feng, Yaoyu[1];Xiao, Lihua[2]

机构：[1]E China Univ Sci & Technol, Sch Resources & Environm Engn, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[2]Ctr Dis Control & Prevent, Div Foodborne Waterborne & Environm Dis, Atlanta, GA USA;[3]New York City Dept Environm Protect, Valhalla, NY USA;[4]Ctr Dis Control & Prevent, Div Parasit Dis & Malaria, Atlanta, GA USA

年份：2014

卷号：80

期号：1

起止页码：218

外文期刊名：APPLIED AND ENVIRONMENTAL MICROBIOLOGY

收录：;EI(收录号：20140117160758);WOS:【SCI-EXPANDED(收录号:WOS:000328851400023)】；

基金：This work was supported by the National Natural Science Foundation of China (grants 31229005 and 31110103901); Fundamental Research Funds for the Central Universities, China; the National Special Fund for the State Key Laboratory of Bioreactor Engineering (no. 2060204); and the Centers for Disease Control and Prevention.

语种：英文

外文关键词：Mammals - Storms

摘要：To assess the host specificity of Enterocytozoon bieneusi and to track the sources of E. bieneusi contamination, we genotyped E. bieneusi in wildlife and stormwater from the watershed of New York City's source water, using ribosomal internal transcribed spacer (ITS)-based PCR and sequence analyses. A total of 255 specimens from 23 species of wild mammals and 67 samples from stormwater were analyzed. Seventy-four (29.0%) of the wildlife specimens and 39 (58.2%) of the stormwater samples from streams were PCR positive. Altogether, 20 E. bieneusi genotypes were found, including 8 known genotypes and 12 new ones. Sixteen and five of the genotypes were seen in animals and stormwater from the watershed, respectively, with WL4 being the most common genotype in both animals (35 samples) and stormwater (23 samples). The 20 E. bieneusi genotypes belonged to five genogroups (groups 1, 3, 4, and 7 and an outlier), with only 23/113 (20.4%) E. bieneusi-positive samples belonging to zoonotic genogroup 1 and 3/20 genotypes ever being detected in humans. The two genogroups previously considered host specific, groups 3 and 4, were both detected in multiple groups of mammals. Thus, with the exception of the type IV, Peru11, and D genotypes, which were detected in only 7, 5, and 2 animals, respectively, most E. bieneusi strains in most wildlife samples and all stormwater samples in the watershed had no known public health significance, as these types have not previously been detected in humans. The role of different species of wild mammals in the contribution of E. bieneusi contamination in stormwater was supported by determinations of host-adapted Cryptosporidium species/genotypes in the same water samples. Data from this study indicate that the host specificity of E. bieneusi group 3 is broader than originally thought, and wildlife is the main source of E. bieneusi in stormwater in the watershed.