文献类型：期刊文献

中文题名：香豆素类比率荧光探针的合成及其对亚硫酸盐与硫化氢的区分检测

英文题名：A Coumarin-based Ratiometric Fluorescent Probe for Differentiating Sulfites and Hydrogen Sulfide

作者：姚余华[1];田海玉[1];裴晓良[1];陈扬[1];张维冰[1];钱俊红[1]

机构：[1]上海市功能性材料重点实验室华东理工大学化学与分子工程学院,上海200237

年份：2018

卷号：37

期号：4

起止页码：397

中文期刊名：分析测试学报

外文期刊名：Journal of Instrumental Analysis

收录：CSTPCD;;北大核心:【北大核心2017】；CSCD:【CSCD2017_2018】；

基金：国家自然科学基金资助项目 (21576085 )

语种：中文

中文关键词：荧光探针;亚硫酸盐;硫化氢;区分检测

外文关键词：fluorescent probe;sulfite;hydrogen sulfide;differentiation

摘要：基于迈克尔加成反应和分子内电荷转移机理设计合成了一个比率型荧光探针MSP,并将其用于对亚硫酸盐和硫化氢的区分检测。强吸电子基团丙二腈的引入,使得探针的吸收和发射波长红移,同时极大地提高了探针与亚硫酸盐的反应速率,实现了对亚硫酸盐的快速检测。当反应时间延长至3 h时,硫化氢的存在使得探针的荧光波长蓝移至510 nm且强度显著增强,探针在两个波长下的荧光比率(I510/I690)增强约260倍,实现了在不同响应时间和不同光谱通道区分检测亚硫酸盐与硫化氢的目的。该探针具有良好的选择性和竞争性,可分别利用吸收强度比值(A464/A572)和荧光强度比值(I510/I690)定量检测亚硫酸盐和硫化氢,两者检出限分别为0.95μmol/L和0.6μmol/L。该探针还能用于对活细胞内亚硫酸盐和硫化氢的荧光成像。
A ratiometric fluorescent probe MSP,based on Michael addition reaction and intramolecular charge transfer mechanism,was fabricated for the differentiated determination on sulfite and hydrogen sulfide.A strong electron with draw group malononitrile was conjugated to coumarin fluorophore,which activated the electrophilic activity of the C ‖ C double bond,and strengthened the electron push-pull effect of the probe.As a result,obvious red-shifts in absorption and emission maxima,and great acceleration of the reaction rate between sulfite and the probe could be expected,which enabled MSP to detect sulfite rapidly.As prolonging the assay time to 3 h,the presence of hydrogen sulfide led to obvious blue-shifts in absorption and emission maxima along with significant fluorescence enhancement,and about 260-fold enhancement of the ratio of fluorescence intensities at 510 nm and 690 nm was observed.It is possible to make distinguishment between sulfite and hydrogen sulfide at different response time and different spectral channels.The probe exhibited excellent selectivity and competition toward sulfite and hydrogen sulfide.Good linear relationships between A 464 / A 572 and sulfite,and I 510 / I 690 and hydrogen sulfide over the concentration ranges of 0-200 μmol/L and 0-500 μmol/L were obtained,respectively.The detection limits for sulfite and sulfide were estimated to be 0.95 μmol/L and 0.6 μmol/L,respectively.In addition,the probe could be used for bioimaging sulfite and hydrogen sulfide in living cells.