文献类型：期刊文献

英文题名：2-D RP/RPLC method to separate components in Fructus schisandrae chinensis

作者：Jin, Gaowa[1,2]; Dai, Yuntao[3]; Feng, Jiatao[1]; Qin, Xuemei[3]; Xue, Xingya[1,4]; Zhang, Feifang[1,4]; Liang, Xinmiao[1,4,5]

机构：[1] Key Lab of Separation Science for Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, China; [2] East China Sea Fishery Research Institute, Chinese Academy of Fishery Sciences, Shanghai, China; [3] Modern Research Center of Traditional Chinese Medicines, Shanxi University, Taiyuan, China; [4] School of Pharmacy, East China University of Science and Technology, Shanghai, China; [5] Dalian Institute of Chemical Physics, Chinese Academy of Sciences, East China University of Science and Technology, 457 Zhongshan Road, Dalian, Liaoning 116023, China

年份：2010

卷号：33

期号：4-5

起止页码：564

外文期刊名：Journal of Separation Science

收录：EI(收录号：20101012762331)

语种：英文

外文关键词：Chromatography - Separation

摘要：An off-line 2-D RP/RPLC method was developed to separate components in extracts of Fructus schisandrae chinensis, which is a traditional Chinese medicine. In this method, Click β-CD stationary phase was proposed for constructing 2-D RP/RPLC system combined with C18 column. β-CD column showed great potential to separate compounds in traditional Chinese medicines under RP mode. Collected fractions from Click β-CD column used in first dimension were resolved into many additional components, indicating that the 2-D LC system based on Click β-CD and C18 phases is orthogonal. The mobile phase was same on both columns. This 2-D LC method facilitates us not only to separate components that cannot be resolved by unidimensional chromatography, but also to achieve much more efficient detection of minor sample components. ? 2010 Wiley-VCH Verlag GmbH & Co. KGaA.