文献类型：期刊文献

英文题名：Enhancement of BMP-2-mediated angiogenesis and osteogenesis by 2-N,6-O-sulfated chitosan in bone regeneration

作者：Pan, Yuanzhong[1,2];Chen, Jie[1,2];Yu, Yuanman[1,2];Dai, Kai[1,2];Wang, Jing[1,2];Liu, Changsheng[1,2,3]

机构：[1]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[2]East China Univ Sci & Technol, Engn Res Ctr Biomed Mat, Minist Educ, Shanghai 200237, Peoples R China;[3]East China Univ Sci & Technol, Minist Educ, Key Lab Ultrafine Mat, Shanghai 200237, Peoples R China

年份：2018

卷号：6

期号：2

起止页码：431

外文期刊名：BIOMATERIALS SCIENCE

收录：;EI(收录号：20180604753105);WOS:【SCI-EXPANDED(收录号:WOS:000423742400019)】；

基金：We express our gratitude for the financial support received from the National Natural Science Foundation of China (No. 31330028, 31470923), International Cooperation Project of Shanghai Science and Technique Committee (15520711100), and Key Project of Logistic Scientific Research of PLA (BWS13C014). This study was also supported by the 111 Project (B14018).

语种：英文

外文关键词：Biosynthesis - Bone - Proteins - Sulfur compounds

摘要：Sulfated polysaccharides are attractive semi-synthesized materials that can be used as a mimic of heparan sulfate to modulate the protein activity and other physiological processes. In this study, we employed sulfated chitosan to enhance the angiogenic capacity of bone morphogenic protein-2 (BMP-2). Bone marrow stromal cells (BMSCs) cultured in a combination of BMP-2 and 2-N, 6-O-sulfated chitosan (SCS) group exhibited a higher cell viability and sprouting ability. The cells also secreted more VEGF and NO. The expression patterns of angiogenic and osteogenic genes were analyzed, and VEGFR2 signaling was found to play a role in the enhancing effect of SCS. The chick embryo chorioallantoic membrane (CAM) assay revealed an enhanced angiogenic effect of BMP-2 when SCS was involved. In addition, we investigated angiogenesis and osteogenesis in mouse using a BMP-2-induced ectopic bone model. Histological and immune-staining analysis revealed that both bone and vascular tissue were enhanced when SCS was added. These data prove that SCS can improve the angiogenic potential of BMP-2 and thus lead to better bone regeneration.