文献类型：期刊文献

英文题名：Microbial Community Analysis of Fresh and Old Microbial Biofilms on Bayon Temple Sandstone of Angkor Thom, Cambodia

作者：Lan, Wensheng[1,2];Li, Hui[1,3];Wang, Wei-Dong[4];Katayama, Yoko[5];Gu, Ji-Dong[1,6]

机构：[1]Univ Hong Kong, Sch Biol Sci, Hong Kong, Hong Kong, Peoples R China;[2]Shenzhen Exit & Entry Inspect & Quarantine Bur, Tech Ctr Anim Plant & Food Inspect & Quarantine, Shenzhen 518001, Peoples R China;[3]E China Univ Sci & Technol, State Environm Protect Key Lab Environm Risk Asse, Shanghai 200237, Peoples R China;[4]Heilongjiang Bayi Agr Univ, Coll Biol Sci & Technol, Daqing 163319, Heilongjiang, Peoples R China;[5]Tokyo Univ Agr & Technol, Grad Sch Agr, Dept Environm & Nat Resource Sci, Fuchu, Tokyo 1838509, Japan;[6]Univ Hong Kong, Swire Inst Marine Sci, Cape Daguilar, Hong Kong, Peoples R China

年份：2010

卷号：60

期号：1

起止页码：105

外文期刊名：MICROBIAL ECOLOGY

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000280701500009)】；

基金：We would like to thank the assistance of Xianshu Li and Asako Kusumi in sampling on site. Support of this research project was provided by Safeguarding of Bayon Temple of Angkor Thom (JASA, Japan), APSARA Authority of Cambodian Government, and the UNESCO/Japanese Funds-in-Trust for the Preservation of the World Cultural Heritage.

语种：英文

摘要：The temples of Angkor monuments including Angkor Thom and Bayon in Cambodia and surrounding countries were exclusively constructed using sandstone. They are severely threatened by biodeterioration caused by active growth of different microorganisms on the sandstone surfaces, but knowledge on the microbial community and composition of the biofilms on the sandstone is not available from this region. This study investigated the microbial community diversity by examining the fresh and old biofilms of the biodeteriorated bas-relief wall surfaces of the Bayon Temple by analysis of 16S and 18S rRNA gene sequences. The results showed that the retrieved sequences were clustered in 11 bacterial, 11 eukaryotic and two archaeal divisions with disparate communities (Acidobacteria, Actinobacteria, Bacteroidetes, Cyanobacteria, Proteobacteria; Alveolata, Fungi, Metazoa, Viridiplantae; Crenarchaeote, and Euyarchaeota). A comparison of the microbial communities between the fresh and old biofilms revealed that the bacterial community of old biofilm was very similar to the newly formed fresh biofilm in terms of bacterial composition, but the eukaryotic communities were distinctly different between these two. This information has important implications for understanding the formation process and development of the microbial diversity on the sandstone surfaces, and furthermore to the relationship between the extent of biodeterioration and succession of microbial communities on sandstone in tropic region.