文献类型：期刊文献

英文题名：Chinese hamster ovary cell performance enhanced by a rational divide-and-conquer strategy for chemically defined medium development

作者：Liu, Yaya[1];Zhang, Weiyan[1];Deng, Xiancun[2];Poon, Hong Fai[2];Liu, Xuping[1];Tan, Wen-Song[1];Zhou, Yan[1];Fan, Li[1]

机构：[1]E China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[2]Hisun Pharmaceut Hangzhou Co Ltd, Hangzhou 311404, Zhejiang, Peoples R China

年份：2015

卷号：120

期号：6

起止页码：690

外文期刊名：JOURNAL OF BIOSCIENCE AND BIOENGINEERING

收录：;EI(收录号：20152801032069);WOS:【SCI-EXPANDED(收录号:WOS:000366765200014)】；

基金：This work was supported by the National Natural Science Foundation of China (Nos. 21106045 and 21206040), the National High Technology Research and Development Program of China (863 Program) (No. 2012AA02A303), the National Science and Technology Major Project (No. 2013ZX10004003-003-003), and the Fundamental Research Funds for the Central Universities (WF1214035).

语种：英文

外文关键词：Basal medium design; Chemical-defined; Chinese hamster ovary; High-yielding; Monoclonal antibody

摘要：Basal medium design is considered one of the most important steps in process development. To optimize chemically defined (CD) media efficiently and effectively for the biopharmaceutical industry, a two-step rational strategy was applied to optimize four antibody producing Chinese hamster ovary (CHO) cell lines. In the first step, 48 of 52 components of our in-house medium were divided into three groups according to their characteristics. In the next step, these groups were optimized by spent medium analysis, response surface methodology and mixture design. Because these steps in our strategy involved dividing medium components into groups and subsequently adjusting the concentration of the components, we termed this medium development strategy "divide and conquer". By applying the strategy, we were able to improve the titers of CHO-S, CHO-DG44 and two CHO-K1 cell lines 1.92, 1.86, 2.92 and 1.62-fold, respectively, in 8 weeks with fewer than 60 tests. This divide-and-conquer strategy was efficient, effective, scalable and universal in our current study and offered a new approach to CD media development. (c) 2015, The Society for Biotechnology, Japan. All rights reserved.