文献类型：期刊文献

英文题名：Improvement of Ansamitocin P-3 Production by Actinosynnema mirum with Fructose as the Sole Carbon Source

作者：Li, Tinglan[1];Fan, Yuxiang[1];Nambou, Komi[1];Hu, Fengxian[1];Imanaka, Tadayuki[1];Wei, Liujing[1];Hua, Qiang[1]

机构：[1]E China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China

年份：2015

卷号：175

期号：6

起止页码：2845

外文期刊名：APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY

收录：;EI(收录号：20151000612244);WOS:【SCI-EXPANDED(收录号:WOS:000350881100006)】；

基金：This study was financially supported by the National Natural Science Foundation of China (Nos. 31200025 and 21406070), and Research Fund for the Doctoral Program of Higher Education of China (20130074110002) and partially supported by the Fundamental Research Funds for the Central Universities of China (WF1214045).

语种：英文

外文关键词：Actinosynnema mirum; Ansamitocin P-3; Fructose; Glucose; Metabolite analysis

摘要：Ansamitocin P-3 (AP-3) is an active and potent anti-tumor maytansinoid, which is usually produced by Actinosynnema spp. In this study, the effects of different carbon sources on biomass and AP-3 production by Actinosynnema mirum were investigated. The results showed great biomass production behavior of A. mirum in glucose medium comparatively to other carbon sources. Interestingly, when fructose was used as the sole carbon source, the highest yield of AP-3 was obtained, which was about fourfold than that of strain cultured in glucose after 168 h. Further analysis conducted in regard to better understanding of such observations in glucose and fructose defined media showed that fructose improves AP-3 production through the stimulation of the key genes of the secondary metabolism pathways. It was concluded that fructose could be a potential carbon source for cost-effective production of AP-3 from an industrial point of view.