文献类型：期刊文献

英文题名：Roles of PKAc1 and CRE1 in cellulose degradation, conidiation, and yellow pigment synthesis in Trichoderma reesei QM6a

作者：Li, Ni[1];Chen, Yumeng[1];Shen, Yaling[1];Wang, Wei[1]

机构：[1]East China Univ Sci & Technol, New World Inst Biotechnol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China

年份：2022

卷号：44

期号：12

起止页码：1465

外文期刊名：BIOTECHNOLOGY LETTERS

收录：;EI(收录号：20224313002452);WOS:【SCI-EXPANDED(收录号:WOS:000870925000002)】；

基金：This work was supported by the Natural Science Foundation of Shanghai, China (No. 22ZR1417600), the National Natural Science Foundation of China (32000050), and Shanghai Agriculture Applied Technology Development Program, China (Grant No. 2021-02-08-00-12-F00758).

语种：英文

外文关键词：Carbon catabolite repressor; Cellulase; Protein kinase A; Secondary metabolism; Trichoderma reesei

摘要：Purpose This study aimed to reveal the roles of the protein kinase A catalytic subunit 1 (pkac1) and carbon catabolite repressor cre1 genes in cellulase production by Trichoderma reesei wild-type strain QM6a. Our strategy might be useful to construct a high-yielding cellulase strain for its wide application. Methods This paper describes cellulase activity, plate conidiation, and yellow pigment synthesis assays of QM6a with the disruption of pkac1 and cre1. Results Deletion of pkac1 (Delta pkac1) had no effect on cellulase production or transcript levels of major cellulase genes in the presence of cellulose. Disruption of cre1 (Delta cre1) resulted in a remarkable increase in cellulase production and expression of the four major cellulase genes. Double disruption of pkac1 and cre1 significantly improved enzyme activity and protein production. The double disruption also resulted in a significant reduction in yellow pigment production and abrogated conidial production. Conclusion Double deletion of pkac1 and cre1 led to increased hydrolytic enzyme production in T. reesei using cellulose as a carbon source.