详细信息
文献类型:期刊文献
中文题名:不同有机氮源对肌苷合成的影响
英文题名:Effect of Different Organic Nitrogen Sources on Inosine Biosynthesis
作者:郭元昕[1];陈双喜[1];刘新星[1];储炬[1];庄英萍[1]
机构:[1]华东理工大学生物反应器工程国家重点实验室,上海200237
年份:2006
卷号:32
期号:11
起止页码:1289
中文期刊名:华东理工大学学报(自然科学版)
外文期刊名:Journal of East China University of Science and Technology
收录:CSTPCD;;EI(收录号:20070310373200);Scopus;北大核心:【北大核心2004】;CSCD:【CSCD2011_2012】;
基金:上海市曙光计划资助项目(01SG28);上海市科委重点科技攻关项目(034319220)
语种:中文
中文关键词:肌苷;发酵;有机氮源;蛋白;核酸
外文关键词:inosine; fermentation; organic nitrogen ; protein ; nucleic acid
摘要:通过研究不同有机氮源对肌苷合成的影响,结合蛋白、核酸及菌体生长的分析测定,结果显示:国产酵母膏A是肌苷发酵的理想氮源。此外,由于国产酵母膏A部分可溶,其水解出的氨基酸的碳骨架可能作为前体物质直接进入糖酵解和三羧酸途径,使得以国产酵母膏A为氮源时肌苷生产期的呼吸熵(RQ)由1.0降至0.6左右,从而促进了肌苷的合成,糖苷转化率由原来的15%提高至29%。
Effect of different organic nitrogen sources on inosine biosysnthesis was investigated through the analysis of proteins, nucleic acids and biomass. Results show that domestic yeast extract A can be the ideal nitrogen source for inosine fermentation. In addition, with its partial solubility, the carbon chain of amino acids hydrolysed from domestic yeast A believed to be the precursors to glycolysis pathway and tricarboxylic acid cycle. Usage of domestic extract A as nitrogen source results in the decrease of the respiration quotient (RQ) value from 1.0 to 0. 6 during the inosine production phase and inosine production is elevated correspondingly. The yield coefficient of inosine to glucose increases from 15% to 29%.
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