文献类型：期刊文献

英文题名：Improving cellulosic ethanol fermentability of Zymomonas mobilis by overexpression of sodium ion tolerance gene ZMO0119

作者：Gao, Xiaochuang[1];Gao, Qiuqiang[1];Bao, Jie[1]

机构：[1]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, 130 Meilong Rd, Shanghai 200237, Peoples R China

年份：2018

卷号：282

起止页码：32

外文期刊名：JOURNAL OF BIOTECHNOLOGY

收录：;EI(收录号：20182705405767);WOS:【SCI-EXPANDED(收录号:WOS:000441281200005)】；

基金：This research was supported by the National High-Tech Program of China (2012AA022301) and the Natural Science Foundation of China (31300070).

语种：英文

外文关键词：Zymomonas mobilis; Cellulosic ethanol; Sodium ion; ZMO0119; Lignocellulose

摘要：Inhibition of sodium ion (Na+) on Zymomonas mobilis represents an important obstacle for efficient cellulosic ethanol production. This study screened and overexpressed the genes responsible for transporting metal ions in Z. mobilis for increasing its Na+ tolerance. The ZMO0119 gene encoding Na+/H+ antiporter was identified to be highly effective for reducing intracellular Na+ concentration of Z. mobilis by improving the Na+ transport capacity. Overexpression of ZMO0119 gene in Z. mobilis significantly accelerated the cell growth, glucose consumption, and cellulosic ethanol production from the dry acid pretreated and biodetoxified corn stover feedstock. This study provided an important gene responsible for increasing the cellulosic ethanol fermentability by Z. mobilis.