文献类型：期刊文献

中文题名：一锅法全细胞催化合成烟酰胺单核苷酸的研究

英文题名：One-pot whole-cell catalytic synthesis of nicotinamide mononucleotides

作者：施玲慧[1];王宇晓[1];任宇红[1]

机构：[1]华东理工大学生物工程学院,生物反应器工程国家重点实验室,上海200237

年份：2023

卷号：43

期号：7

起止页码：182

中文期刊名：现代化工

外文期刊名：Modern Chemical Industry

收录：CSTPCD;;Scopus;北大核心:【北大核心2020】；CSCD:【CSCD_E2023_2024】；

语种：中文

中文关键词：烟酰胺单核苷酸;全细胞催化;一锅法合成;融合表达;共表达

外文关键词：nicotinamide mononucleotide;whole-cell catalysis;one-pot synthesis;fusion expression;co-expression

摘要：对以腺苷酸(Adenosine monophosphate,AMP)为底物合成β-烟酰胺单核苷酸(β-Nicotinamide mononucleotide,β-NMN)的途径进行了研究。在大肠杆菌(Escherichia coli)BL21(DE3)中构建AMP核苷酶(AMN)与磷酸核糖焦磷酸合成酶(PRS)的共表达及融合表达体系,其中,融合菌株PRS-L4-AMN与烟酰胺磷酸核糖转移酶(NAMPT)构成的双菌催化体系具有较高的催化效率,是三菌混合催化体系的2.42倍。进一步对全细胞催化合成NMN的反应体系进行优化,包括pH、温度、缓冲液、金属离子及菌粉投加量等。结果表明,在优化条件下,反应2 h时NMN产量达到最高,为23.7 mmol/L,总收率达33.4%,实现了NMN的一锅法生物合成。
The pathway for the synthesis ofβ-nicotinamide mononucleotide(β-NMN)using adenosine monophosphate(AMP)as a substrate is investigated.The co-expression and fusion expression systems for AMP nucleosidase AMN and ribose-phosphate pyrophosphokinase(PRS)are constructed within Escherichia coli BL21(DE3).Of which,the dual bacterial catalytic system composing of fusion strain PRS-L4-AMN and nicotinamide phosphoribosyl transferase(NAMPT)shows a high catalytic efficiency,which is 2.42 times that of the mixed three-bacterial catalytic system.The reaction system for the whole-cell catalytic synthesis of NMN is further optimized,including pH,temperature,buffer,metal ions and bacterial powder dosage,etc.Under the optimized conditions,the highest NMN production,23.7 mmol·L-1,is obtained when the reaction has performed for 2 h with a total yield of 33.4%,which realizes the one-pot biosynthesis of NMN.