文献类型：期刊文献

中文题名：杂交瘤细胞流加培养中葡萄糖和谷氨酰胺的代谢控制

英文题名：METABOLIC CONTROL OF GLUCOSE AND GLUTAMINE IN FED-BATCH CULTURES OF HB 58 HYBRIDOMA CELLS

作者：张淑香[1];李东晓[1];朱明龙[1];周燕[1];谭文松[1];张嗣良[1]

机构：[1]华东理工大学生物反应器工程国家重点实验室,上海200237

年份：2004

卷号：55

期号：2

起止页码：247

中文期刊名：化工学报

外文期刊名：CIESC Journal

收录：CSTPCD;;EI(收录号：2004258221640);Scopus;北大核心:【北大核心2000】；CSCD:【CSCD2011_2012】；

语种：中文

中文关键词：杂交瘤细胞;流加培养;葡萄糖限制;谷氨酰胺限制

外文关键词：Cell culture;Glucose;Metabolism

摘要：对底物限制的流加培养中杂交瘤细胞HB 5 8的生长、代谢和单抗生成进行了研究。葡萄糖或谷氨酰胺限制引起比生长速率下降。葡萄糖限制时 ,乳酸生成减少 ,细胞对葡萄糖的得率增加 ,乳酸对葡萄糖的得率降低 ,说明葡萄糖更多地参与三羧酸循环。谷氨酰胺限制时 ,氨和丙氨酸生成减少 ,细胞和氨对谷氨酰胺的得率增加 ,丙氨酸对谷氨酰胺的得率减小 ,说明谷氨酰胺更多地参与脱氢反应 ,其有效利用率得到提高。
Substrate limited fed batch cultures were used to study the growth, metabolism and antibody production of HB 58 hybridoma cells. Glucose or glutamine limitation decreased specific growth rate. In the condition of glucose limitation, lactate formation and YLac/Glc decreased, but Yceii/Gic increased, which suggested that more glucose took part in TCA cycle. In the condition of glutamine limitation, the formation of ammonia and alanine and YAla/Gin decreased, but Ycen/Gin and YAmm/Gln increased, which indicated that more glutamine was incorporated into dehydrogenation reaction and its effective utilization was achieved.