文献类型：期刊文献

英文题名：Separation and purification of verticine and verticinone from Bulbus Fritillariae Thunbergii by high-speed counter-current chromatography coupled with evaporative light scattering detection

作者：Liu, Zhilan[1]; Jin, Yan[1]; Shen, Pingniang[2]; Wang, Juan[2]; Shen, Yongjia[1]

机构：[1] Laboratory for Advanced Materials, Institute of Fine Chemicals, East China University of Science and Technology, Shanghai, 200237, China; [2] National Engineering Research Center for Traditional Chinese Medicine, Shanghai, 201203, China

年份：2007

卷号：71

期号：5

起止页码：1873

外文期刊名：Talanta

收录：EI(收录号：20071110486635)

语种：英文

外文关键词：Chromatographic analysis - Hydrochloric acid - Light scattering - Nuclear magnetic resonance spectroscopy - Purification

摘要：High-speed counter-current chromatography (HSCCC) coupled with evaporative light scattering detection (ELSD) was successfully applied to preparative separation and purification of verticine and verticinone from crude extracts of Bulbus Fritillariae Thunbergii by a one-step separation, using chloroform-ethanol-0.2 mol L-1 hydrochloric acid (3:2:2, v/v/v) as a solvent system. HPLC analysis of the fractions collected on the preparative HSCCC of 200 mg of crude extracts showed that the purity of verticine (25.6 mg) was 96.8% and that of verticinone (10.3 mg) was 95.4%. The chemical identities of these components were confirmed by 1H NMR and EI-MS. ? 2006 Elsevier B.V. All rights reserved.