文献类型：期刊文献

英文题名：Immobilization of lipase on chemically modified bimodal ceramic foams for olive oil hydrolysis

作者：Huang, Lei[1];Cheng, Zhen-Min[1]

机构：[1]E China Univ Sci & Technol, State Key Lab Chem Engn, Shanghai 200237, Peoples R China

年份：2008

卷号：144

期号：1

起止页码：103

外文期刊名：CHEMICAL ENGINEERING JOURNAL

收录：;EI(收录号：20083611525605);WOS:【SCI-EXPANDED(收录号:WOS:000260039900014)】；

基金：This research work was financially supported by the New-Century Excellent Talent Program Supported by Chinese Ministry of Education (NCET-04-0412).

语种：英文

外文关键词：Enzyme immobilization; Ceramic foam; Structured catalyst; Lipase; Carriers

摘要：The work reported in this paper is aimed at exploring the feasibility of immobilizing alkali lipase from Penicillium expansum on a bimodal ceramic foam, which has both macro- and micro-pore structures. After being chemically modified with a silane coupling agent, the ceramic foam was used as a support for lipase immobilization. To determine the preferable immobilization conditions, the effects of the amount of enzyme for loading, immobilization time, temperature, and pH on enzyme activity were investigated. The results showed that the chemically modified ceramic foam has a high loading capacity and a strong binding ability for the lipase. Thanks to the low internal mass transfer resistance, the ceramic foam has greatly enhanced the rate of immobilization. As a comparison, the immobilized-lipase activity was much higher than that on many frequently used porous materials like diatomite, alumina and activated carbon. (C) 2008 Elsevier B.V. All rights reserved.