文献类型：期刊文献

英文题名：Calcineurin signaling pathway influences Aspergillus niger biofilm formation by affecting hydrophobicity and cell wall integrity

作者：Liu, Li[1,2];Yu, Bin[1,2];Sun, Wenjun[1,2];Liang, Caice[1,2];Ying, Hanjie[1,2,3];Zhou, Shengmin[4];Niu, Huanqing[1,2];Wang, Yibing[4];Liu, Dong[1,2,3];Chen, Yong[1,2]

机构：[1]Nanjing Tech Univ, Coll Biotechnol & Pharmaceut Engn, Natl Engn Res Ctr Biotechnol, Nanjing, Peoples R China;[2]Nanjing Tech Univ, State Key Lab Mat Oriented Chem Engn, Coll Biotechnol & Pharmaceut Engn, Nanjing 211816, Peoples R China;[3]Zhengzhou Univ, Sch Chem Engn & Energy, Zhengzhou 450001, Peoples R China;[4]East China Univ Sci & Technol, Sch Biotechnol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China

年份：2020

卷号：13

期号：1

外文期刊名：BIOTECHNOLOGY FOR BIOFUELS

收录：;EI(收录号：20201508386655);WOS:【SCI-EXPANDED(收录号:WOS:000521251800003)】；

基金：This work was supported by the key program of the National Natural Science Foundation of China (Grant No. 21636003), the Outstanding Youth Foundation of Jiangsu (Grant No. SBK2017010373), the National Key Research and Development Program of China (Grant Nos. 2018YFA0902200, 2018YFB1501705), the Program for Changjiang Scholars and Innovative Research Team in University (IRT_14R28), Jiangsu National Synergetic Innovation Center for Advanced Materials (SICAM), the Technology Support Program of Jiangsu (Grant No. BE2014715), and the Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD).

语种：英文

外文关键词：Aspergillus niger; Biofilm; Calcineurin signaling pathway; Cell wall integrity; Hydrophobicity

摘要：Background Biofilms, as a kind of fixed-cell community, can greatly improve industrial fermentation efficiency in immobilized fermentation, but the regulation process is still unclear, which restricts their application. Ca2+ was reported to be a key factor affecting biofilm formation. However, the effect of Ca2+ on biofilm structure and microbiology was yet only studied in bacteria. How Ca2+-mediated calcineurin signaling pathway (CSP) alters biofilm formation in bacteria and fungi has rarely been reported. On this basis, we investigated the regulation of CSP on the formation of biofilm in Aspergillus niger. Results Deletion of the key genes MidA, CchA, CrzA or CnaA in the CSP lowered the Ca2+ concentration in the mycelium to a different extent, inhibited the formation of A. niger biofilm, reduced the hydrophobicity and adhesion of spores, destroyed the cell wall integrity of hyphae, and reduced the flocculation ability of hyphae. qRT-PCR results showed that the expression of spore hydrophobic protein RodA, galactosaminogalactan (GAG) biosynthesis genes (uge3, uge5, agd3, gtb3), and alpha-1,3-glucan biosynthesis genes (ags1, ags3) in the increment MidA, increment CchA, increment CrzA, increment CnaA strains were significantly down-regulated compared with those of the wild type (WT). In addition, the transcription levels of the chitin synthesis gene (chsB, chsD) and beta-1,3-glucan synthesis gene (FksA) were consistent with the change in chitin and beta-1,3-glucan contents in mutant strains. Conclusion These results indicated that CSP affected the hydrophobicity and adhesion of spores, the integrity of mycelial cell walls and flocculation by affecting Ca2+ levels in mycelium, which in turn affected biofilm formation. This work provides a possible explanation for how CSP changes the formation of A. niger biofilm, and reveals a pathway for controlling biofilm formation in industrial immobilized fermentation.