文献类型：期刊文献

英文题名：Pyrethrum-extract induced autophagy in insect cells: A new target?

作者：Xu, Zhiping[1];Lu, Miaoqing[1];Yang, Mingjun[1];Xu, Wenping[1];Gao, Jufang[2];Zhang, Yang[1];Yang, Yun[1];Tao, Liming[1]

机构：[1]East China Univ Sci & Technol, Shanghai Key Lab Chem Biol, Sch Pharm, Shanghai 200237, Peoples R China;[2]Shanghai Normal Univ, Coll Life & Environm Sci, Shanghai 200234, Peoples R China

年份：2017

卷号：137

起止页码：21

外文期刊名：PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000399067000004)】；

基金：This work was financial supported by National Key Technology Research Development Program of China (No. 2011BAEO6B04).

语种：英文

外文关键词：Insecticide; Pyrethrum-extract; Sf9 cells; Cytotoxicity; Autophagy

摘要：Pyrethrum extract (PY) is a natural insecticide that is extensively used across the world, and its insecticidal activity is attributed to the presence of six active esters known as pyrethrins. PY targets the nervous systems of insects by delaying the closure of voltage-gated sodium ion channels in the nerve cells. However, limited information is available regarding the toxicity and detailed mechanisms of PY activity. This study is aimed at understanding the toxicity effect and the underlying mechanisms of PY in cellular level, which have not yet been investigated on the non-nervous system of insects. Results of the MIT assay showed that the viability of SIB cells was inhibited by PY in a time- and concentration-dependent manner, and observation under a microscope revealed accumulation of intracellular vacuoles. Monodansylcadaverine staining analysis and transmission electron microscope images revealed typical autophagic morphological changes in PY-treated SIB cells. Autophagy-related proteins such as LC3, p62, and beclin-1 were detected using by Western blotting. Protein expression levels of LC3-II and beclin-1 were upregulated while that of p62 was markedly downregulated in a dose-dependent manner upon the PY treatment in Sf9 cells. In conclusion, these results indicate that PY could induce autophagy in the non-nervous system of insects which may contribute to its insecticidal mechanism. (C) 2016 Elsevier Inc. All rights reserved.