文献类型：期刊文献

英文题名：Qualitative and quantitative analysis of an alkaloid fraction from Piper longum L. using ultra-high performance liquid chromatography-diode array detector-electrospray ionization mass spectrometry

作者：Li, Kuiyong[1];Fan, Yunpeng[1];Wang, Hui[1];Fu, Qing[1];Jin, Yu[1];Liang, Xinmiao[1]

机构：[1]E China Univ Sci & Technol, Sch Pharm, Minist Educ, Engn Res Ctr Pharmaceut Proc Chem, Shanghai 200237, Peoples R China

年份：2015

卷号：109

起止页码：28

外文期刊名：JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000353078700004)】；

基金：This work was supported by the Natural Science Foundation of Shanghai, China (Grant No. 13ZR1453200) and the Specialized Research Fund for the Doctoral Program of Higher Education of China (Grant No. 20130074120017).

语种：英文

外文关键词：Qualitative; Quantitative; Alkaloid fraction; Piper longum L.; Ultra-high performance liquid chromatography-diode array detector-electrospray ionization mass spectrometry (UHPLC-DAD-MS)

摘要：In a previous research, an alkaloid fraction and 18 alkaloid compounds were prepared from Piper longum L. by series of purification process. In this paper, a qualitative and quantitative analysis method using ultrahigh performance liquid chromatography-diode array detector-mass spectrometry (UHPLC-DAD-MS) was developed to evaluate the alkaloid fraction. Qualitative analysis of the alkaloid fraction was firstly completed by UHPLC-DAD method and 18 amide alkaloid compounds were identified. A further qualitative analysis of the alkaloid fraction was accomplished by UHPLC-MS/MS method. Another 25 amide alkaloids were identified according to their characteristic ions and neutral losses. At last, a quantitative method for the alkaloid fraction was established using four marker compounds including piperine, pipernonatine, guineensine and N-isobutyl-2E,4E-octadecadienamide. After the validation of this method, the contents of above four marker compounds in the alkaloid fraction were 57.5 mg/g, 65.6 mg/g, 17.7 mg/g and 23.9 mg/g, respectively. Moreover, the relative response factors of other three compounds to piperine were calculated. A comparative study between external standard quantification and relative response factor quantification proved no remarkable difference. UHPLC-DAD-MS method was demonstrated to be a powerful tool for the characterization of the alkaloid fraction from P. longum L. and the result proved that the quality of alkaloid fraction was efficiently improved after appropriate purification. (C) 2015 Published by Elsevier B.V.