文献类型：期刊文献

英文题名：Staurosporine shows insecticidal activity against Mythimna separata Walker (Lepidoptera: Noctuidae) potentially via induction of apoptosis

作者：Zhang, Yang;Liu, Songlin[1];Yang, Xing[2];Yang, Mingjun[1];Xu, Wenping[1];Li, Yaxiao[3];Tao, Liming[1]

机构：[1]E China Univ Sci & Technol, Sch Pharm, Shanghai Key Lab Chem Biol, Shanghai 200237, Peoples R China;[2]Shanghai Pesticide Res Inst, Shanghai 200032, Peoples R China;[3]Univ Bath, Dept Biol & Biochem, Bath BA2 7AY, Avon, England

年份：2016

卷号：128

起止页码：37

外文期刊名：PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000372679700006)】；

基金：This work was financially supported by the National Key Technology Research Development Program of China (863 Program, NO. 2011AA10A202-2) and (NO. 2011BAE06B04).

语种：英文

外文关键词：Insecticidal activity; Staurosporine; Mythimna separata Walker; Apoptosis; Sf9 cells; The mitochondrial pathway

摘要：Staurosporine (STS), a wide-spectrum kinase inhibitor, is widely used in studies of apoptosis in mammalian cells. However, its physiological and mechanistic effects have never been clearly defined in insect cells, and other applications of STS have rarely been reported. The present study reveals the insecticidal activity of STS on larvae of Mythimna separata Walker, and the apoptotic mechanism induced by STS on lepidopteran Sf9 cell lines. We demonstrate that the viability of Sf9 cells is inhibited by STS in a time- and concentration-dependent manner. Intracellular biochemical assays show that STS-induced apoptosis of Sf9 cells coincides with a decrease in the mitochondrial membrane potential, the release of cytochrome c into the cytosol, a significant increase of the Bax/Bcl-2 ratio, and a marked activation of caspase-9 and caspase-3. These results indicate that a mitochondrial-dependent intrinsic pathway contributes to STS induced caspase-3 activation and apoptosis in Sf9 cells which is homologous to the mechanisms in mammalian cells. This study contributes to our understanding of the mechanism of insect cell apoptosis and suggests a possible new application of STS as a potential insecticide against Lepidopteran insect pests in agriculture. (C) 2015 Elsevier Inc. All rights reserved.