文献类型：期刊文献

中文题名：动物细胞培养过程中pH和溶氧的关联控制

英文题名：Interactive Control of pH and DO in the Animal Cell Culture

作者：顾小华[1];陈因良[1]

机构：[1]华东化工学院生化工程研究所

年份：1989

卷号：15

期号：4

起止页码：498

中文期刊名：华东化工学院学报

收录：国家哲学社会科学学术期刊数据库;Scopus;CSCD:【CSCD2011_2012】；

语种：中文

中文关键词：细胞培养;动物细胞;pH;溶氧

外文关键词：cell culture; control systems; compensation networks; sequential control; interactive control of pH and DO; compensating calculation

摘要：本文通过对动物细胞培养过程中pH值和溶氧(DO)水平控制要求的分析,设计了用空气、氧气、氮气和二氮化碳四组气体关联控制pH值和DO水平的控制系统。该系统根据对瞬时pH值和DO值参数的检测,通过微处理机进行一系列的逻辑判断和具有PI调节规律的DDC增量词节计算,再经四气体关联互补计算和多重时序控制环节,综合调节四组气体电磁阀的相互开闭时间,实现对培养过程中pH值和DO水平的控制,满足动物细胞生长的需要。
A new design of interactive control scheme is presented for the regulation of pH and DO in the mass culture of animal cells. It is simply composed of four-gas (air, oxygen, carbon dioxide and nitrogen) coupling compensating calculation (CCC) and multiple-sequence-control (MSC). By a series of logic judgements of signals from sensors and direct digital calculations (DDC), a simple proportional plus integral (PI) controller can manipulate the operations of electrocegnetic valves to meet the requirements of pH and DO levels in the growth and metabolism of animal cells.