文献类型：期刊文献

英文题名：Sequential heterotrophy-dilution-photoinduction cultivation for efficient microalgal biomass and lipid production

作者：Fan, Jianhua[1];Huang, Jianke[1];Li, Yuanguang[1];Han, Feifei[1];Wang, Jun[2];Li, Xinwu[2];Wang, Weiliang[1];Li, Shulan[2]

机构：[1]E China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[2]Shanghai Zeyuan Marine Biotechnol Ltd Co, Shanghai 200237, Peoples R China

年份：2012

卷号：112

起止页码：206

外文期刊名：BIORESOURCE TECHNOLOGY

收录：;EI(收录号：20121414925902);WOS:【SCI-EXPANDED(收录号:WOS:000302971200029)】；

基金：We thank Dr. Chunzhao Liu and Dr. Jian Xu for critical discussions and for reading the manuscript. This research was supported by the National Natural Science Foundation of China 21076080, National Basic Research Program of China 2011CB200904 and, National Key Technologies R&D Program 2011BAD14B02 and 2011BAD23B04 National Special Fund for State Key Laboratory of Bioreactor Engineering 2060204.

语种：英文

外文关键词：Microalgae; Heterotrophy-Dilution-Photoinduction; Chlorella; Biomass; Lipid

摘要：A novel cultivation strategy called "sequential heterotrophy-dilution-photoinduction" was developed for efficient algal biomass and lipid production. Three Chlorella species were first cultivated heterotrophically to achieve high cell density, then the broth was diluted to suitable concentration (2-5 g/L) and transferred to light environment for photoinduction. With this strategy, the Chlorella intracellular protein and chlorophyll increased rapidly to 50.87% and 32.97 mg/g by a 12-h illumination, which were close to the level of cells cultivated photoautotrophically. Moreover, the lipid contents were increased by 84.57%, 70.65% and 121.59% within 24-h photoinduction for C vulgaris, C. pyrenoidosa and C. ellipsoidea, respectively. Maximum lipid content as 26.11% of biomass and maximum lipid productivity of 89.89 mg/L/d was both accomplished by C. pyrenoidosa. Further outdoor experiments showed consistent patterns. Therefore, the proposed strategy provided an effective approach for microalgal biomass production to meet the urgent need for both health food and biodiesel. (C) 2012 Elsevier Ltd. All rights reserved.