文献类型：期刊文献

中文题名：铵离子对林可霉素发酵过程的动态影响

英文题名：Dynamic Effects of Ammonium Ions on Fermentation of Lincomycin

作者：李啸[1];储炬[1];张嗣良[1];杭海峰[1];庄英萍[1]

机构：[1]华东理工大学生物工程学院,国家生物反应器重点实验室,上海200237

年份：2008

卷号：39

期号：5

起止页码：339

中文期刊名：中国医药工业杂志

外文期刊名：Chinese Journal of Pharmaceuticals

收录：CSTPCD;;北大核心:【北大核心2004】；CSCD:【CSCD2011_2012】；

基金：上海市科委重点科技攻关项目(064319033)

语种：中文

中文关键词：林可链霉菌;林可霉素;铵离子;发酵;生物合成

外文关键词：Streptomyces lincolnensis; lincomycin; ammonium ion; fermentation; biosynthesis

摘要：在林可链霉菌(Streptomyces lincolnensis)发酵生产林可霉素的过程中,经分析表明:补入硫酸铵前,应待发酵液中的NH4+浓度消耗至1.0mmol/L以下,使迟效碳、氮源充分降解,可能促使林可霉素合成酶大量合成;20～24h,尽可能提高硫酸铵的平均补入速率(最高瞬时浓度<19.0mmol/L),以缩短生物量的积累时间;24h后逐渐降低NH4+的平均补入速率,使主代谢流转入次级代谢;40h后将摄氧率和最低NH4+浓度分别控制在20～30mmol·(L·h)-1和3.0～4.0mmol/L,每24h生物效价的涨幅达1000μg/ml以上。
Through analysis in the course of fermentation of lincomycin by Streptomyces lincolnensis, it was found that ammonium ions should be consumed below 1.0mmol/L before feeding ammonium sulfate to the fermentation broth, so that slow-released carbon and nitrogen source might be degraded completely and large amount of lincomycin synthase might be synthesized at the same time. In the period of fermentation 20 - 24h, the average rate of feeding ammonium sulfate should be increased （the highest instantaneous concentration ofNH4^＋〈 19.0mmol/L） so as to shorten the time for biomass accumulation. After 24h, the average rate of feeding ammonium sulfate should be decreased gradually in order to make the main metabolism flow divert to secondary metabolism flow, after 40h oxygen uptake rate and concentration of NH4^＋ should be controlled at 20 - 30mmol· （L·h）^ -1 and 3.0 - 4.0mmol/L, resepectively. The increase scope oftiter could reach to above 1000μg/ml every 24h.