文献类型：期刊文献

英文题名：Combined heat and exercise stress disrupt gut microbiota and promote microbial translocation

作者：Min, Leizi[1,2];Ablitip, Alimjan[2];Wang, Qingyuan[2];Li, Ting[3];Di, Qian[4,5];Ma, Xindong[2,6];Fang, Wen[1]

机构：[1]East China Univ Sci & Technol, Sch Sports Sci, Shanghai, Peoples R China;[2]Tsinghua Univ, Div Sports Sci & Phys Educ, Beijing, Peoples R China;[3]East China Univ Sci & Technol, Sch Pharm, Shanghai, Peoples R China;[4]Tsinghua Univ, Inst Hlth China, Beijing, Peoples R China;[5]Tsinghua Univ, Vanke Sch Publ Hlth, Beijing, Peoples R China;[6]Tsinghua Univ, IDG McGovern Inst Brain Res, Beijing, Peoples R China

年份：2026

卷号：17

外文期刊名：FRONTIERS IN MICROBIOLOGY

收录：;Scopus(收录号：2-s2.0-105044340239);WOS:【SCI-EXPANDED(收录号:WOS:001786358700001)】；

基金：The author(s) declared that financial support was received for this work and/or its publication. This research was supported by the Shanghai Municipal Education Science Research Planning Project (No. A2025003), the Special Project for Central Education Reform of China (No. JGS02262001), the cooperative research project between East China University of Science and Technology and Anhui Sanlian University (No. L110-72581), the National Key Research and Development Program of China (2023YFA1802003 to TL), NSFC (32571513 to TL), and the Natural Science Foundation of Shanghai (24ZR1415800 to TL).

语种：英文

外文关键词：bacterial translocation; exertional heat stroke; gut microbiota; heat stress; intestinal barrier

摘要：Purpose The incidence of exertional heat stroke (EHS) has increased markedly in recent decades. Although intestinal barrier dysfunction and gut microbiota alterations are increasingly implicated in EHS pathophysiology, the respective contributions of heat exposure and physical exercise to these processes remain incompletely defined.Methods Male C57BL/6 mice were assigned to Control (C), Exercise (E), Heat shock (H), or Exercise + Heat shock (HE) groups. Exercise and/or heat exposure were applied to induce exertional heat stress. Intestinal injury and permeability were assessed by histopathology and circulating D-lactate levels. Gut and blood microbial profiles were characterized using 16S rRNA gene sequencing, and associations between microbial signatures and intestinal injury markers were analyzed.Results Both heat exposure and exercise induced intestinal injury and increased circulating D-lactate levels, with the most severe effects observed in the combined HE group. Heat exposure was associated with pronounced alterations in gut microbial diversity and community structure, whereas exercise was associated with increased microbial diversity and gut-associated microbial signatures detected in blood samples. Differential abundance analyses revealed distinct taxonomic profiles associated with heat, exercise, and their combination. Correlation analyses demonstrated significant associations between intestinal injury markers and circulating microbial profiles.Conclusion These findings indicate that heat exposure and exercise exert distinct yet interacting associations with intestinal barrier integrity and microbial community distribution. Heat stress primarily disrupts gut microbial ecology and barrier function, whereas exercise is more closely associated with increased systemic detection of gut-derived microbial signatures. Together, these results highlight the gut microbiota-barrier axis as a key interface linking environmental and physiological stressors to systemic responses during exertional heat stress.