文献类型：期刊文献

中文题名：Lysinibacillus sp.Re Cr-13还原Cr(Ⅵ)特性及对Cr(Ⅵ)诱导的氧化胁迫响应

英文题名：Cr(Ⅵ) reduction by Lysinibacillus sp. ReCr-13 and Cr(Ⅵ)-induced oxidative stress

作者：邱永秋[1];吴炜进[1];刘浩[1];李西燕[1];张旭[1];朱明龙[1];谭文松[1]

机构：[1]华东理工大学生物反应器工程国家重点实验室

年份：2019

卷号：33

期号：4

起止页码：856

中文期刊名：高校化学工程学报

外文期刊名：Journal of Chemical Engineering of Chinese Universities

收录：CSTPCD;;Scopus;北大核心:【北大核心2017】；CSCD:【CSCD2019_2020】；

基金：国家自然科学基金(21878083)

语种：中文

中文关键词：Lysinibacillus;sp.;ReCr-13;Cr(Ⅵ)还原;氧化胁迫;Mn2+;抗氧化

外文关键词：Lysinibacillus sp.ReCr-13;Cr(Ⅵ) reduction;oxidative stress;Mn2+;antioxidant

摘要：含Cr(Ⅵ)工业废水对环境造成严重污染。一些微生物能够将Cr(Ⅵ)还原为低毒性的Cr(Ⅲ)。从河道污泥中筛选到一株Cr(Ⅵ)还原菌并鉴定为Lysinibacillus sp. ReCr-13。实验探究了该菌还原Cr(Ⅵ)的特性及其对Cr(Ⅵ)诱导的氧化胁迫响应。在pH值为8.0、NaCl为5 g×L^-1、Cr(Ⅵ)为200 mg×L^-1条件下,Lysinibacillus sp. ReCr-13具有最佳的铬还原效率。Cr(Ⅵ)浓度在200~400 mg×L^-1,随初始Cr(Ⅵ)浓度的增加,胞内O2^·-、H2O2及MDA的含量增加,然而Cr(Ⅵ)还原效率下降。外加Mn2+能够有效促进胞内抗氧化酶SOD和CAT的活性,能降低O2^·-、H2O2及MDA的含量,并最终提高了Cr(Ⅵ)还原效率。研究结果将为生物还原含Cr(Ⅵ)工业废水提供一定的理论基础。
Chromium-containing industrial wastewater causes serious pollution problems, while certain microorganism can reduce Cr(Ⅵ) to less toxic Cr(Ⅲ). A Cr(Ⅵ) reducing strain was isolated from river sludge and identified as Lysinibacillus sp. ReCr-13. Cr(Ⅵ) bio-reduction of the strain and oxidative stress response induced by Cr(Ⅵ) were investigated. The optimum Cr(Ⅵ) reduction efficiency was achieved by Lysinibacillus sp. ReCr-13 at pH 8.0, NaCl 5 g×L^-1 and 200 mg×L^-1 Cr(Ⅵ). The contents of intracellular O2^·-, H2O2 and MDA increase with the increase of initial Cr(Ⅵ) concentration in the range of 200~400 mg×L^-1,while the reduction efficiency of Cr(Ⅵ) decreases. The exogenous addition of Mn^2+ can effectively promote the activity of intracellular antioxidant enzymes SOD and CAT, reduce the contents of intracellular O2^·-, H2O2 and MDA, and ultimately improve the reduction efficiency of Cr(Ⅵ). The results provide fundamental information for Cr(Ⅵ)bio-reduction in chromium-containing industrial wastewater.