文献类型：期刊文献

英文题名：Astaxanthin accumulation difference between non-motile cells and akinetes of Haematococcus pluvialis was affected by pyruvate metabolism

作者：Fang, Lei[1];Zhang, Jingkui[1];Fei, Zhongnan[1];Wan, Minxi[1]

机构：[1]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, Mail Box 301,Meilong Rd 130, Shanghai 200237, Peoples R China

年份：2020

卷号：7

期号：1

外文期刊名：BIORESOURCES AND BIOPROCESSING

收录：;EI(收录号：20224513081457);WOS:【SCI-EXPANDED(收录号:WOS:000511704600001)】；

基金：This research was funded by National Natural Science Foundation of China (31500062), China, Post Doctoral Science Foundation (2013M530183 and 2014T70400), China, and the Fundamental Research Funds for the Central Universities (222201414024), China. The authors declared that they have no conflicts of interest to this work.

语种：英文

外文关键词：Astaxanthin; Haematococcus pluvialis; Pyruvate; Transcriptome

摘要：Background Haematococcus pluvialis is the best source of natural astaxanthin, known as the king of antioxidants. H. pluvialis have four cell forms: spore, motile cell, non-motile cell and akinete. Spores and motile cells are susceptible to photoinhibition and would die under photoinduction conditions. Photoinduction using non-motile cells as seeds could result in a higher astaxanthin production than that using akinetes. However, the mechanism of this phenomenon has not been clarified. Results Transcriptome was sequenced and annotated to illustrate the mechanism of this phenomenon. All differentially expressed genes involved in astaxanthin biosynthesis were up-regulated. Particularly, chyb gene was up-regulated by 16-fold, improving the conversion of beta-carotene into astaxanthin. Pyruvate was the precursor of carotenoids biosynthesis. Pyruvate kinase gene expression level was increased by 2.0-fold at the early stage of akinetes formation. More changes of gene transcription occurred at the early stage of akinetes formation, 52.7% and 51.9% of total DEGs in control group and treatment group, respectively. Conclusions Genes transcription network was constructed and the synthesis mechanism of astaxanthin was clarified. The results are expected to further guide the in-depth optimization of the astaxanthin production process in H. pluvialis by improving pyruvate metabolism.