文献类型：期刊文献

英文题名：Multiple and sensitive SERS detection of cancer-related exosomes based on gold-silver bimetallic nanotrepangs

作者：Ning, Cui-Fang[1];Wang, Luying[1];Tian, Ya-Fei[2];Yin, Bin-Cheng[1,2,3];Ye, Bang-Ce[1,2,3]

机构：[1]Zhejiang Univ Technol, Inst Engn Biol & Hlth, Collaborat Innovat Ctr Yangtze River Delta Reg Gr, Coll Pharmaceut Sci, Hangzhou 310014, Zhejiang, Peoples R China;[2]East China Univ Sci & Technol, Lab Biosyst & Microanal, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[3]Shihezi Univ, Sch Chem & Chem Engn, Xinjiang 832000, Peoples R China

年份：2020

卷号：145

期号：7

起止页码：2795

外文期刊名：ANALYST

收录：;EI(收录号：20201508390969);WOS:【SCI-EXPANDED(收录号:WOS:000526825700036)】；

基金：This work was jointly supported by the National Natural Science Foundation of China (Grant 21822402, 21675052, 31730004), the National Key Research and Development Project (2018YFA0900404), and China Postdoctoral Science Foundation (ZY19014148002).

语种：英文

外文关键词：Gold - Nanorods - Biomarkers - Diagnosis - Diseases - Probes

摘要：Exosomes are endogenous vesicles of cells, and can be used as important biomarkers for cancers. In this work, we developed a sensitive and reliable SERS sensor for simultaneous detection of multiple cancer-related exosomes. The SERS detection probes were made of bimetallic SERS-active nanotags, gold-silver-silver core-shell-shell nanotrepangs (GSSNTs), which were composed of bumpy surface nanorod (gold nanotrepang, GNT) cores and bilayer silver shells, and decorated with linker DNAs, which were complementary to the aptamer targeting exosomes. Three kinds of SERS detection probes were designed via the adoption of different Raman reporter molecules and linker DNAs. The capture probes were prepared by modifying specific aptamers of the target exosomes on magnetic beads (MBs). In the absence of target exosomes, SERS detection probes were coupled with MBs via specific DNA hybridization for use as aptamer-based SERS sensors. In the presence of target exosomes, the aptamer specifically recognized and captured the exosomes, and GSSNTs were subsequently released into the supernatant. Therefore, attenuated SERS signals were detected on the MBs, indicating the presence of target exosomes. The proposed aptamer-based SERS sensor is expected to be a facile and sensitive method for the multiplex detection of cancer biomarkers and has potential future applications in clinical diagnosis.