文献类型：期刊文献

英文题名：Adjuvant contributes Roundup's unexpected effects on A549 cells

作者：Hao, Youwu[1];Zhang, Yang[1];Cheng, Jiagao[1];Xu, Wenping[1];Xu, Zhiping[1];Gao, Jufang[2];Tao, Liming[1]

机构：[1]East China Univ Sci & Technol, Sch Pharm, Shanghai Key Lab Chem Biol, Shanghai 200237, Peoples R China;[2]Shanghai Normal Univ, Coll Life Sci, Shanghai 200234, Peoples R China

年份：2020

卷号：184

外文期刊名：ENVIRONMENTAL RESEARCH

收录：;EI(收录号：20242616394575);WOS:【SCI-EXPANDED(收录号:WOS:000526178000006)】；

基金：This work was financial supported by National Key Research and Development Plan (2017YFD0200300) and Shanghai Sailing Program (NO. 19YF1411100).

语种：英文

外文关键词：Roundup; Adjuvant; Apoptosis; DNA damage; POEA

摘要：Roundup (R) (RDP) is one of the most representative glyphosate-based herbicides (GBHs), which extensive use increases pressure on environmental safety and potential human health risk. The aim of this study was to investigate whether the adjuvant polyethoxylated tallow amine (POEA) or the herbicidal active ingredient glyphosate isopropylamine salt (GP) in formulation confers RDP cytotoxicity. We demonstrated that RDP and POEA could inhibit the proliferation of human lung A549 cells. Intracellular biochemical assay indicated that collapse of mitochondrial membrane, release of cytochrome c into cytosol, activation of caspase-9/-3, cleavage of poly (ADP-ribose) polymerase (PARP), oxidative DNA damage, DNA single-strand breaks and double-strand breaks are occurred in RDP and POEA treated A549 cells, not occurred in GP treated A549 cells. We conclude that the RDP's effect of apoptosis and DNA damage on human A549 cells is related to the presence of adjuvant POEA in formulation, independent of the herbicidal active ingredient GP. This study would enrich the theoretical basis of the RDP toxicity effects and attract attention on potential human health and environmental safety threat caused by adjuvant.