文献类型：期刊文献

英文题名：Chemical Synthesis and Immunological Evaluation of Escherichia coli O29, Shigella dysenteriae Serotype 11, and Proteus vulgaris O12 O-Antigen Oligosaccharides

作者：Li, Xinxin[1];Wang, Xiaotong[2];Deng, Lei[1];Ji, Xiang[2];Tu, Yuanhong[1];Liu, Can[1];Wang, Zhen[1];Wang, Liming[1];Ye, Xinshan[3,4];Yang, You[2];Zhang, Qingju[1,5]

机构：[1]Jiangxi Normal Univ, Coll Chem & Mat, Natl Engn Res Ctr Carbohydrate Synth, Nanchang, Peoples R China;[2]East China Univ Sci & Technol, Shanghai Frontiers Sci Ctr Optogenet Tech Cell Met, Engn Res Ctr Pharmaceut Proc Chem, Shanghai Key Lab New Drug Design, Shanghai, Peoples R China;[3]Peking Univ, Sch Pharmaceut Sci, State Key Lab Nat & Biomimet Drugs, Beijing, Peoples R China;[4]Peking Univ, Chem Biol Ctr, Beijing, Peoples R China;[5]Jiangxi Normal Univ, Jiangxi Prov Key Lab Nat & Biomimet Drugs Res, Nanchang, Peoples R China

年份：2026

外文期刊名：ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

收录：;EI(收录号：20262620978341);WOS:【SCI-EXPANDED(收录号:WOS:001799560700001)】；

基金：Dedicated to Professor Peter H. Seeberger on the occasion of his 60th birthday. This work was financially supported by the National Natural Science Foundation of China (22167015, 22577027, 22377043, 22407053), the Science and Technology Department of Jiangxi Province (20244BDF60014, JXSQ2020101084, 20224ACB213006, 20252BAC220023, and 2024SSY07051), the Natural Science Foundation of Shanghai (25ZR1401088), and the Shanghai Frontiers Science Center of Optogenetic Techniques for Cell Metabolism (Shanghai Municipal Education Commission).

语种：英文

外文关键词：broad-spectrum vaccine; carbohydrate-based vaccine; E. coli O29; oligosaccharide synthesis; P. vulgaris O12

摘要：Escherichia coli (E. coli), Shigella dysenteriae (S. dysenteriae), and Proteus vulgaris (P. vulgaris) are the leading bacterial causes of death and diseases in many countries. Vaccines are economical and effective tools against bacterial infection. Herein, we present the first total synthesis and immunological evaluation of the E. coli O29, S. dysenteriae serotype 11, and P. vulgaris O12 O-antigen decasaccharides, pentasaccharides, and related oligosaccharide fragments. We found that the hexasaccharide, including two repeating units of the core trisaccharide, was the optimal epitope, which was conjugated with the carrier protein CRM197 to furnish a semisynthetic glycoconjugate vaccine. The glycoconjugate vaccine induced a robust antigen-specific immune response, and the antibody sera could recognize the surface O-antigens of E. coli O29. This glycoconjugate vaccine, including a common trisaccharide fragment, existed in many bacterial O-antigens, which can be further used as a potential broad-spectrum vaccine against E. coli, S. dysenteriae, P. vulgaris, and Cronobacter malonaticus (C. malonaticus).