文献类型：期刊文献

英文题名：Hg²⁺-mediated aggregation of gold nanoparticles for colorimetric screening of biothiols

作者：Xu, Hu[1];Wang, Yiwen[1];Huang, Xiaomei[1];Li, Yan[1];Zhang, Hua[1];Zhong, Xinhua[1]

机构：[1]E China Univ Sci & Technol, Shanghai Key Lab Funct Mat Chem, Dept Chem, Shanghai 200237, Peoples R China

年份：2012

卷号：137

期号：4

起止页码：924

外文期刊名：ANALYST

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000299476300020)】；

基金：We thank the National Natural Science Foundation of China (No. 21175043), the Science and Technology Commission of Shanghai Municipality (No. 11JC1403100 and 10dz2220500), the Fundamental Research Funds for the Central Universities, and the Program for Professor of Special Appointment at Shanghai Institutions of Higher Learning for financial supports.

语种：英文

摘要：In this work, we report a colorimetric assay for the screening of biothiols including glutathione (GSH), cysteine (Cys), and homocysteine (Hcys) based on Hg2+-mediated aggregation of gold nanoparticles (AuNPs). Hg2+ can induce aggregation of thiol-containing naphthalimide (1) capped AuNPs due to the cross-linking interactions from the resulting "thymine-Hg2+-thymine'' (T-Hg2+-T) analogous structure. When Hg2+ is firstly treated with biothiols, followed by mixing with 1-capped AuNPs suspension, AuNPs undergo a transformation from an aggregation to a dispersion state depending on the concentration of biothiols. This anti-aggregation or re-dispersion of AuNPs is due to the higher affinity of Hg2+ for biothiols relative to compound 1. The corresponding color variation in the process of anti-aggregation of AuNPs can be used for the quantitative screening of biothiols through UV-vis spectroscopy or by the naked eye. Under optimized conditions, a good linear relationship in the range of 0.025-2.28 mu M is obtained for GSH, 0.035-1.53 mu M for Cys, and 0.040-2.20 mu M for Hcys. The detection limits of this assay for GSH, Cys, and Hcys are 17, 9, and 18 nM, respectively. This colorimetric assay exhibits a high selectivity and sensitivity with tunable dynamic range. The proposed method has been successfully used in the determination of total biothiol content in human urine samples.