文献类型：期刊文献

英文题名：Influence of temperature on nucleus degradation of 4-androstene-3, 17-dione in phytosterol biotransformation by Mycobacterium sp.

作者：Xu, X. W.[1];Gao, X. Q.[1];Feng, J. X.[1];Wang, X. D.[1];Wei, D. Z.[1]

机构：[1]E China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China

年份：2015

卷号：61

期号：1

起止页码：63

外文期刊名：LETTERS IN APPLIED MICROBIOLOGY

收录：;EI(收录号：20231113722732);WOS:【SCI-EXPANDED(收录号:WOS:000355881800010)】；

基金：This article is supported by National Natural Science Foundation of China (No. 21276083) and Natural Science Foundation of China (No. 21276084).

语种：英文

外文关键词：3-ketosteroid-9-hydroxylase; 3-ketosteroid-(1)-dehydrogenase; AD and; or ADD production; biodegradation; Mycobacterium; phytosterol; temperature effect

摘要：One of the steroid intermediates, 4-androstene-3, 17-dione (AD), in the biotransformation of phytosterols is valuable for the production of steroid medicaments. However, its degradation during the conversion process is one of the main obstacles to obtain high yields. In this study, the effect of temperature on nucleus degradation during microbial biotransformation of phytosterol was investigated. The results indicated that microbial degradation of phytosterol followed the AD-ADD-9-OH-ADD' pathway, and that two important reactions involved in nucleus degradation, conversions of AD to ADD and ADD to 9-OH-ADD, were inhibited at 37 degrees C. With a change in the culture temperature from 30 to 37 degrees C, nucleus degradation was reduced from 399% to 176%, due to inhibition of the putative KstD and Ksh. These results suggested a simple way to decrease the nucleus degradation in phytosterol biotransformation and a new perspective on the possibilities of modifying the metabolism of strains used in industrial applications. Significance and Impact of the StudyNucleus degradation of products is one of the main problems encountered during phytosterol biotransformation. To solve this problem, the effect of temperature on nucleus degradation was investigated in the industrial production of steroid intermediates. The results are also helpful to the genetic modification of sterol-producing strains.