文献类型：期刊文献

英文题名：The Retention Behavior of Ginsenosides in HPLC and Its Application to Quality Assessment of Radix Ginseng

作者：Hu, Ping[1];Luo, Guo-An[1,3];Wang, Qing[1];Zhao, Zhong-Zhen[2];Wang, Wan[1];Jiang, Zhi-Hong[2]

机构：[1]E China Univ Sci & Technol, Sch Chem & Mol Engn, Shanghai 200237, Peoples R China;[2]Hong Kong Baptist Univ, Sch Chinese Med, Kowloon Tong, Hong Kong, Peoples R China;[3]Tsinghua Univ, Anal Ctr, Beijing 100084, Peoples R China

年份：2008

卷号：31

期号：10

起止页码：1265

外文期刊名：ARCHIVES OF PHARMACAL RESEARCH

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000260302300006)】；

基金：This research was financially supported by the Research Grants Council, University Grants Committee of Hong Kong (HKBU 2130/04M), and the Scientific Research Plan Programme of the Shanghai Science and Technology Committee of China (05DZ19709).

语种：英文

外文关键词：Ginsenoside; HPLC; Retention behavior; Radix Ginseng; Quantitation; Panax

摘要：This study systematically investigated the retention behavior of seven neutral ginsenosides Rg(1), Re, Rf, Rb-1, Rb-2, Rc, Rd, and an acidic ginsenoside R-0, the major pharmacologically active components of Radix Ginseng with RP-HPLC. The effects of solvent, pH value, ionic strength of the mobile phase, and column temperature were investigated using an octadecylsiloxane-bonded silica gel column. Based on the ginsenosides' retention characteristics, the concentration of acetonitrile and the gradient of the mobile phase needed to maintain the baseline separation of the major neutral ginsenosides in Radix Ginseng were theoretically predicted. Furthermore, the ionic strength of mobile-phase necessary to achieve good resolution of the neutral ginsenosides and acidic ginsenosides was carefully investigated. According to the results of the quantitative analysis of ginsenosides in eight batches of ginseng samples from different sources, the developed HPLC technique may be a valuable tool for the quality assessment of Radix Ginseng.