文献类型：期刊文献

中文题名：不同分子量壳多糖对植物病菌的拮抗作用及其诱导提高寄主植物抗病性

英文题名：Bacteriostatic effect of chitosans with different molecular weights to phytopathogenic fungi and inducing defense responses

作者：张文清[1,2];夏玮[1];程俞[3];张元兴[2]

机构：[1]华东理工大学化学系;[2]华东理工大学生物反应器工程国家重点实验室,上海200237;[3]上海市农药研究所

年份：2004

卷号：31

期号：3

起止页码：235

中文期刊名：植物保护学报

外文期刊名：Journal of Plant Protection

收录：CSTPCD;;北大核心:【北大核心2000】；CSCD:【CSCD2011_2012】；

基金：国家"863"项目(2002AA90240);上海市科技兴农重点攻关项目沪农科攻字(2003)第7-7号

语种：中文

中文关键词：分子量;壳多糖;植物病菌;拮抗作用;寄主植物;抗病性

外文关键词：chitosan;;phytopathogenic fungi;;peroxidase;;β-1,3 glucanase

摘要：采用固体培养基体外抑菌法研究了分子量为1002、2350、5060的三种壳多糖对22种植物病原菌的拮抗作用。结果表明:三种壳多糖对供试植物病原菌有不同程度的抑制作用,随壳多糖浓度的增加对不同病原菌的抑制作用增强,壳多糖的分子量对植物病菌的抑制作用没有显著影响。壳多糖对活体植株染病的防治效果与壳多糖的使用次数呈正相关,水溶性壳多糖(分子量为2350)对活体植株染病的防治效果最好。壳多糖可诱导植物产生与抗病有关的过氧化物酶和β-1,3葡聚糖酶,而这些酶的产生随着处理次数的增加依次增加,分子量为2350的壳多糖诱导作用最佳,可使过氧化物酶活性增加4倍以上,β-1,3葡聚糖酶活性增加2倍以上。
Bacteriostatic effect of chitosans with different molecular weights(1002,2350,5060)to 22 species of phytopathogenic fungi was studied in vitro with solid medium.The re-sults showed that phytopathogenic fungi could be inhibited to different degrees by chitosans.The bacteriostatic effect increased when the concentration of chitosan increasing.The molecularweight of chitosan had little effect on bacteriostatic effect to phytopathogenic fungi.The bacteri-ostatic effect of chitosan for plant was related to treatment times.Chitoosaccharide with 2350had the maximum bacteriostatic effect.Peroxidase and β-1,3 glucanase related-defense respon-ses were induced by chitosan.The activities of these enzymes were increased when treatmenttimes increasing.Chitosan with 2350 had the maximum induced activity,and made the activityof peroxidase increase 4 times and the activity of β-1,3 glucanase increase 1 time.