文献类型：期刊文献

英文题名：A novel ratiometric fluorescent probe based on an internal reference of lanthanide/nucleotide for alkaline phosphatase detection

作者：Yu, Haoran[1];Qiang, Ziqing[1];Sun, Yelin[1];Sun, Mengyao[1];Zhang, Lei[1];Yu, Bohao[1];Lei, Wen[2];Zhang, Weibing[1]

机构：[1]East China Univ Sci & Technol, Sch Chem & Mol Engn, Shanghai Key Lab Funct Mat Chem, Shanghai 200237, Peoples R China;[2]Shanghai Res Inst Chem Ind CO LTD, 345 East Yunling Rd, Shanghai 200062, Peoples R China

年份：2024

卷号：150

期号：1

起止页码：87

外文期刊名：ANALYST

收录：;EI(收录号：20244817447031);WOS:【SCI-EXPANDED(收录号:WOS:001362798400001)】；

基金：This work was financially supported by the Scientific Instrumentation Program of Shanghai Science and Technology Innovation Action Plan (21142201600), the National Key R&D Program of China (No. 2021YFF0701900), the National Natural Science Foundation of China (21707034), and the Science and Technology Commission of Shanghai Municipality (No. 20142200900).

语种：英文

外文关键词：Adenosinetriphosphate - Body fluids - Encapsulation - Phosphatases - Phosphorylation - Silica nanoparticles - SiO2 nanoparticles

摘要：Based on the specific hydrolytic ability of alkaline phosphatase (ALP), a novel biocompatible ratiometric lanthanide fluorescent probe based on an internal reference (CIP@SiO2-Ce/ATP-Tris) was constructed with Ce3+ as the central ion, adenosine triphosphate (ATP) as the ligand, Tris as the auxiliary ligand and ciprofloxacin (CIP) encapsulated into SiO2 nanoparticles as the reference signal. The fluorescent probe emits characteristic fluorescence at 363 nm belonging to Ce3+ as the working signal and at 435 nm belonging to CIP as the reference signal. Dephosphorylation disrupted the coordination of Ce/ATP-Tris with the enzymatic reaction of ALP, which resulted in fluorescence quenching of Ce3+. The reference fluorescence was kept stable because of the protective effect of encapsulation by SiO2. The biosensor analysis method was achieved by comparing the relationship between I435/I363 and ALP concentration. The detection limit is 0.0025 U L-1, and the linear range of detection is 0.1-20 U L-1. It was subsequently used to detect ALP in samples of fetal bovine serum and human serum, and promising results were obtained.