文献类型：期刊文献

英文题名：Simultaneous quantification of urinary purines and creatinine by ultra high performance liquid chromatography with ultraviolet spectroscopy and quadrupole time-of-flight mass spectrometry: Method development, validation, and application to gout study

作者：Yuan, Yuan[1];Jiang, Min[2];Zhang, Hongyang[1,3];Liu, Ju[2];Zhang, Min[3];Hu, Ping[1]

机构：[1]East China Univ Sci & Technol, Sch Chem & Mol Engn, Shanghai 200237, Peoples R China;[2]Jiujiang First Peoples Hosp, Dept Rheumatol, Jiujiang, Peoples R China;[3]East China Univ Sci & Technol, Sch Pharm, Shanghai Key Lab New Drug Design, Shanghai, Peoples R China

年份：2019

卷号：42

期号：15

起止页码：2523

外文期刊名：JOURNAL OF SEPARATION SCIENCE

收录：;EI(收录号：20192607094432);WOS:【SCI-EXPANDED(收录号:WOS:000478080800007)】；

基金：This research was funded by the National Natural Science Foundation of China (No. 81202493), the Opening Project of Shanghai Key Laboratory of New Drug Design (No. 17DZ2271000), and the Science and Technology Project of Jiangxi Municipal Commission of Health and Family Planning (No. 20177079). We sincerely thank Dr. Yuerong Wang of East China University of Science and technology for her technical assistance in UPLC-UV-Q-TOF-MS experiments.

语种：英文

外文关键词：creatinine; gout; human urine; purine metabolites; purines

摘要：We present an ultra high performance liquid chromatography with ultraviolet spectroscopy and quadrupole time-of-flight mass spectrometry method for the simultaneous quantification of ten purines (adenine, hypoxanthine, guanine, xanthine, deoxyadenosine, adenosine, inosine, guanosine, xanthosine, and uric acid) and creatinine in human urine. After chromatographic separation on an ACE Excel 2 AQ column, high abundant creatinine and uric acid and the other low abundant purines were sequentially detected by ultraviolet and quadrupole time-of-flight mass spectrometry within a single run. Method validations including specificity (improved by accurate mass measurement), linearity (correlation coefficients >= 0.9944), limit of quantification (0.002-9.756 mu g/mL), intra- and interday precision (relative standard deviations <= 9.1 and 14.0%, respectively), accuracy (relative errors <= 13.1%), extraction recovery (between 90.3 and 109.6%), matrix effect (between 85.3 and 110.5%), and stability (relative errors <= 14.3%) were fully evaluated. This approach was applied to characterize the disordered purine metabolism in acute and chronic gout as an example. Quantitative results (normalized by creatinine) showed that an overproduction of urinary purine precursors might be involved in the gout process. The developed method represents a useful tool to investigate the purine disturbances in gout and other relevant diseases.