文献类型：期刊文献

英文题名：Potential threat of Chlorpyrifos to human liver cells via the caspase-dependent mitochondrial pathways

作者：Zhang, Yang[1];Chang, Yuansen[1];Cao, Haijing[1];Xu, Wenping[1];Li, Zhong[1];Tao, Liming[1]

机构：[1]East China Univ Sci & Technol, Sch Pharm, Shanghai Key Lab Chem Biol, Shanghai 200237, Peoples R China

年份：2018

卷号：29

期号：1

起止页码：294

外文期刊名：FOOD AND AGRICULTURAL IMMUNOLOGY

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000435923200001)】；

基金：This work was financially supported by the National Key Technology Research Development Program of China [grant number 2011BAE06B04].

语种：英文

外文关键词：Pesticide; Chlorpyrifos; human liver cells; cytotoxic effects; the mitochondrial apoptosis pathway

摘要：Chlorpyrifos (CPF) has been widely used around the world as a pesticide for both agricultural and residential application. Although various studies have reported toxicity and health-related effects from CPF exposure, the potential threat and the molecular mechanism of CPF toxicity to human liver have not been well-characterized. In this study, we identify cytotoxicity of CPF to human normal liver cells in vitro. We demonstrate that the viability of QSG7701 cells is inhibited by CPF in a time- and concentration-dependent manner. Intracellular biochemical assays showed that CPF-induced apoptosis of QSG7701 cells concurrent with a decrease in the mitochondrial membrane potential, the release of cytochrome c into the cytosol, up-regulate the expression level of Bax/Bcl-2 and a marked activation of caspase-9/-3. These results indicate that CPF has a potential risk to human liver that can induce apoptosis of human liver cells through caspase-dependent mitochondrial pathways.