文献类型：期刊文献

英文题名：Investigating electron-transfer processes using a biomimetic hybrid bilayer membrane system

作者：Ma, Wei[1];Ying, Yi-Lun[1];Qin, Li-Xia[1];Gu, Zhen[1];Zhou, Hao[1];Li, Da-Wei[1];Sutherland, Todd C.[2];Chen, Hong-Yuan[3];Long, Yi-Tao[1]

机构：[1]E China Univ Sci & Technol, Sch Chem & Mol Engn, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[2]Univ Calgary, Dept Chem, Calgary, AB T2N 1N4, Canada;[3]Nanjing Univ, Dept Chem, Key Lab Analyt Chem Life Sci, Nanjing 210008, Peoples R China

年份：2013

卷号：8

期号：3

起止页码：439

外文期刊名：NATURE PROTOCOLS

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000317110600001)】；

基金：This research was supported by the National Science Fund for Distinguished Young Scholars (21125522), the Major Research Plan of the National Natural Science Foundation of China (91027035), the Fundamental Research Funds for the Central Universities (WK1013002) and the 973 Program (2013CB733700).

语种：英文

摘要：Here we report a protocol to investigate the electron-transfer processes of redox-active biomolecules in biological membranes by electrochemistry using biomimetic hybrid bilayer membranes (HBMs) assembled on gold electrodes. Redox-active head groups, such as the ubiquinone moiety, are embedded in HBMs that contain target molecules, e. g., nicotinamide adenine dinucleotide (NADH). By using this approach, the electron-transfer processes between redox molecules and target biomolecules are mediated by mimicking the redox cycling processes in a natural membrane. Also included is a procedure for in situ surface-enhanced Raman scattering (SERS) to confirm the electrochemically induced conformational changes of the target biomolecules in the HBMs. In addition, each step in constructing the HBMs is characterized by electrochemical impedance spectroscopy (EIS), high-resolution X-ray photoelectron spectroscopy (XPS) and atomic force microscopy (AFM). The time required for the entire protocol is similar to 12 h, whereas the electrochemical measurement of electron-transfer processes takes less than 1 h to complete.