文献类型：期刊文献

中文题名：富氧条件下活性氧和初始Fe^(2+)浓度对难处理金精矿生物氧化过程的影响

英文题名：Effects of Reactive Oxygen and Fe^(2+) Concentration on Biooxidation of Refractory Gold Concentrates under Oxygen-Rich Conditions

作者：王红[1];王兆印[1];张旭[1];朱明龙[1];谭文松[1]

机构：[1]华东理工大学生物反应器工程国家重点实验室,上海200237

年份：2016

卷号：30

期号：1

起止页码：104

中文期刊名：高校化学工程学报

外文期刊名：Journal of Chemical Engineering of Chinese Universities

收录：CSTPCD;;EI(收录号：20161102091916);Scopus;北大核心:【北大核心2014】；CSCD:【CSCD2015_2016】；

基金：国家高技术研究发展计划(863计划)项目(2007AA060904;2012AA061503)

语种：中文

中文关键词：难处理金精矿;生物氧化;溶氧;氧化自由基;Fe^2+浓度

外文关键词：refractory gold concentrates; biooxidation; dissolved oxygen; reactive oxygen species（ROS）; Fe^2＋ concentration;

摘要：提高难处理金精矿生物氧化过程中的矿石氧化效率、缩短氧化周期是当前亟待解决的问题。氧是生物氧化过程中的电子受体,矿浆中Fe^(2+)可为铁氧化细菌提供能量,氧气和Fe^(2+)的充足供应将有利于提高菌株的生物氧化活性,而富氧条件下(氧得到充足供应)冶金菌株对能源物质Fe^(2+)的需求尚未见公开报道。今在实验室1.5 L通气搅拌釜式反应器中,利用以L.ferriphilum和S.thermosulfidooxidans为主的混合浸矿菌对黄铁矿包裹的难处理金精矿进行生物氧化,深入研究溶氧水平(DO)和矿浆初始Fe^(2+)浓度对生物氧化过程效率的影响,研究结果发现,当溶解氧水平提高时,生物氧化效率会呈现先升后降的趋势,且随DO水平提高,ROS含量明显上升。富氧条件下(4.0 ppm)随着初始Fe^(2+)浓度的提高,冶金菌株氧化活性在过程初期受到抑制,后期得到增强,ROS含量随初始Fe^(2+)浓度的提高不断增加,而菌体生长却一直受到抑制,最终导致矿石氧化效率的降低。
Adequate supply of oxygen as a final electron acceptor was essential for biological oxidation processes, and Fe^2＋ in pulp can provide energy for iron oxidizing bacteria and help to improve their biooxidation activity. However, the demand for Fe^2＋ of biooxidation strain under oxygen-rich conditions has not been reported publicly. In this study, a mixed bioleaching bacteria strain（L. ferriphilum and S. thermosulfidooxidans） was used to pretreat refractory gold concentrates in a 1.5L laboratory stirred tank reactor. The effects of dissolved oxygen（DO） and Fe^2＋ concentration on the biooxidation process were investigated. The results show that biooxidation efficiency increases at first and then decreases as the DO level elevated. In addition, the ROS content within the bacterial cells significantly increases as the DO level increased. With the increase of initial Fe^2＋ concentration under oxygen-rich conditions, the oxidative activity of the microbial group is inhibited in the beginning and enhanced later and the content of ROS increases, but the cell growth is inhibited throughout the whole process, which leads to the decrease of biooxidation efficiency.