文献类型：期刊文献

英文题名：Identification of an Imine Reductase for Asymmetric Reduction of Bulky Dihydroisoquinolines

作者：Li, Hao[1];Tian, Ping[2];Xu, Jian-He[1];Zheng, Gao-Wei[1]

机构：[1]East China Univ Sci & Technol, Shanghai Collaborat Innovat Ctr Biomfg, State Key Lab Bioreactor Engn, 130 Meilong Rd, Shanghai 200237, Peoples R China;[2]Chinese Acad Sci, Shanghai Inst Organ Chem, 345 Lingling Rd, Shanghai 200032, Peoples R China

年份：2017

卷号：19

期号：12

起止页码：3151

外文期刊名：ORGANIC LETTERS

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000403854300029)，CCR-EXPANDED(收录号:WOS:000403854300029)】；

基金：This work was financially supported by the National Natural Science Foundation of China (Nos. 31200050, 21472045, and 21536004), the Fundamental Research Funds for the Central Universities (22A201514043) and Shanghai Pujiang Program (15PJ1401200).

语种：英文

摘要：A new imine reductase from Stackebrandtia nassauensis (SnIR) was identified, which:displayed over 25- to 1400-fold greater catalytic efficiency for 1-methyl-3,4-dihydroisoquinoline (1-Me DHIQ) compared to other imine reductases reported. Subsequently, an efficient SnIR-catalyzed process was developed by simply optimizing the amount of cosolvent, to 15 g L-1 1-Me DHIQ was converted: completely without a feeding strategy. Furthermore, the: reaction proceeded well for a panel of dihydroisoquinolines, affording the corresponding tetrahydroisoquinolines (mostly in S-configuration) in good yields, (up to 81%) and with moderate to excellent enantioselectivities (up to 99% ee).