文献类型：期刊文献

中文题名：根癌农杆菌介导透明颤茵血红蛋白基因(vgb)在短密青霉菌中的克隆表达

英文题名：Cloning and Expression of vgb Gene in Penicillium Brevicompactum by Agrobacterium Tumefaciens-mediates Transformation(ATMT)

作者：董玉国[1];王丽华[1];徐瑞[1];张舰[1];孙爱友[1];魏东芝[1]

机构：[1]生物反应器工程国家重点实验室,华东理工大学生物工程学院,上海200237

年份：2015

卷号：29

期号：1

起止页码：133

中文期刊名：高校化学工程学报

外文期刊名：Journal of Chemical Engineering of Chinese Universities

收录：CSTPCD;;EI(收录号：20151300692438);Scopus;北大核心:【北大核心2014】；CSCD:【CSCD2015_2016】；

基金：国家科技重大专项资助(2012ZX09304009)

语种：中文

中文关键词：根癌农杆菌;短密青霉;vgb;霉酚酸;发酵

外文关键词：Agrobacterium tumefaciens;Penicillium Brevicompactum;vgb;myhophenoilc acid;fermentation

摘要：利用根癌农杆菌LBA4404介导,将编码透明颤菌血红蛋白的结构基因(Vitreoscilla Hemoglobin gene,vgb)导入到短密青霉ATCC16024中,用于改善菌体对氧的摄取能力,提高霉酚酸(Myhophenoilc Acid,MPA)的发酵水平,降低生产成本。通过潮霉素抗性筛选、PCR分子鉴定和MPA发酵验证等结果表明,vgb基因在短密青霉中成功获得了表达;vgb基因的表达提高了短密青霉的菌体密度,转化子MPA产量达到2.18 g·L?1,比原始菌株提高了27.5%。
Agrobacterium tumefaciens-mediates transformation(ATMT)was used to express the Vitreoscilla Hemoglobin gene(vgb)in Penicillium Brevicompactum ATCC160224.The effects of cloning vgb gene in ATCC16024 on the oxygen utilization and the myhophenoilc acid(MPA)production were studied.Results show that vgb gene was successfully inserted into the transformant genome,which was confirmed by hygromycin-resistance selection,PCR and fermentation experiments.The growth rate and cell density of transformants are higher than control.The MPA production reaches 2.18 g·L^-1,which is a 27.5%increase compared to the host strain.