文献类型：期刊文献

中文题名：介孔硅基干凝胶的降解性及其生物相容性研究

英文题名：Degradability and Biocompatibility of Mesoporous Silica-based Xerogel

作者：李晓生[1];刘昌胜[1];洪华[1];戴程隆[1];吴文静[1]

机构：[1]华东理工大学超细材料制备与应用教育部重点实验室,教育部医用生物材料工程研究中心,上海200237

年份：2008

卷号：24

期号：12

起止页码：1928

中文期刊名：无机化学学报

外文期刊名：Chinese Journal of Inorganic Chemistry

收录：CSTPCD;;Scopus;WOS:【SCI-EXPANDED(收录号:WOS:000262217800002)】；北大核心:【北大核心2004】；CSCD:【CSCD2011_2012】；

基金：国家自然科学基金(No.20425621);上海市科委纳米专项基金(No.0652nm021)资助项目。

语种：中文

中文关键词：硅基干凝胶;降解;比表面;介孔;细胞培养

外文关键词：silica-based xerogel; degradation; specific surface area; mesopore; cell cultivation

摘要：本研究利用改进的溶胶-凝胶工艺合成了介孔硅基干凝胶。通过差热-热重(DTA-TG)、X-射线衍射(XRD)、红外光谱(FTIR)和N2等温吸附-脱附测试分析,以及扫描电镜(SEM)、透射电镜(TEM)观察,表征了硅基干凝胶的热效应、相态、结构、形貌特征,并对合成的硅基干凝胶进行了体外降解与细胞培养实验研究。测试结果表明:合成的硅基干凝胶为平均孔径3nm左右、最大比表面积901m2·g-1的介孔结构。体外模拟体液(Simulated body fluid,SBF)降解结果显示,低温处理的材料在42d内完全降解,直线拟合符合一级反应动力学方程。通过添加钙磷成分或高温煅烧处理可以调控干凝胶的降解速率,使硅基干凝胶的降解呈现直线式匀速降解的特征。体外细胞培养表明:硅基干凝胶可促进C2C12细胞(C3H小鼠肌原细胞)的分化和繁殖,证明材料的生物相容性良好。
The mesoporous silica-based xerogel had been synthesized using improved sol-gel method. The thermal effect, crystalline state, structure and morphology characters of xerogel were measured by DTA-TG, XRD, FITR, SEM, TEM and N2 adsorption-desorption analysis. The degradation characterization and the cellular behavior were investigated by degradation test in simulated body fluid （SBF） and cultivation of cells, respectively. The results indicate that the synthesized xerogel was mesoporous material. The mean diameter of pores and the largest specific surface area of the xerogel were 3 nm and 901 m^2·g^-1, respectively. The low-temperature treated xerogel degraded entirely within 42 days, and degradation simulating curves was in accordance with one-order dynamics reactive equation. The xerogels degradation velocity was controlled at a relative low ratio, and degradation manner transformed into steady linearity character by sintering processes at high temperature, as well as adding calcium and phosphate ingredients into silica-based xeroel. The xerogel promoted the differentiation and conglomeration of C2Cl2 cells （C3H mice muscle cells）, which revealed good biocompatibility of the material.