文献类型：期刊文献

英文题名：A ratiometric fluorescent probe for sensitive, selective and reversible detection of copper (II) based on riboflavin-stabilized gold nanoclusters

作者：Zhang, Min[1];Huynh-Nhu Le[1];Jiang, Xiao-Qin[1];Guo, Su-Miao[1];Yu, Hai-Jun[1];Ye, Bang-Ce[1]

机构：[1]E China Univ Sci & Technol, State Key Lab Bioreactor Engn, Lab Biosyst & Microanal, Shanghai 200237, Peoples R China

年份：2013

卷号：117

起止页码：399

外文期刊名：TALANTA

收录：;EI(收录号：20134316879567);WOS:【SCI-EXPANDED(收录号:WOS:000328801400059)】；

基金：This study is supported by the China NSF 21075040, the Shanghai Fund (11nm0502500, 11XD1401900), SRFDP (No. 20120074110009) and the Key Grant Project (No. 313019) of Chinese Ministry of Education, and the Fundamental Research Funds for the Central Universities.

语种：英文

外文关键词：Gold nanoclusters; Riboflavin; Fluorescence; Ratiometric probe; Copper (II) detection

摘要：Most of the copper (II) fluorescent probes are based on the measurement of fluorescence at a single wavelength, which may be influenced by variations in the sample environment. To the end, the ratiometric fluorescent measurement, which involves the simultaneous measurement of two fluorescence signals at different wavelengths followed by calculation of their intensity ratio, can effectively eliminate the adverse effects on fluorescence signals and give greater precision to the data analysis relative to single-channel detection. In this work, we prepared novel luminescent gold nanoclusters (AuNCs) utilizing vitamin B-2 (riboflavin) as stabilizer by a simple, rapid and one-pot green (low-toxicity materials use) procedure. The as-prepared riboflavin-AuNCs (Ri-AuNCs) solution can be luminescent exhibiting two fluorescence emission peaks at 530 nm and around 840 nm with excitation at 375 nm, however, in the presence of Cu2+, the fluorescence of the Ri-AuNCs was found to be quenched at around 840 nm and enhanced at 530 nm by Cu2+. The resultant ratiometric fluorescent response can provide a novel sensory probe for the determination of Cu2+. The present probe had excellent selectivity in the presence of several cations. The probe revealed a detection limit of 0.9 mu M of Cu2+. Moreover, our proposed probe can reversibly switch between the "on" and "off" states through the addition of Cu2+ and EDTA, which is reusable in practical application. Results and method reported here provide a unique strategy for performance of ratiometric assays demonstrated with a AuNCs-based fluorescent probe, which expands the application of AuNCs. (C) 2013 Elsevier B.V. All rights reserved.