文献类型：期刊文献

英文题名：Enzyme-free amplified SERS immunoassay for the ultrasensitive detection of disease biomarkers

作者：Zhang, Xuan-Xuan[1,2];Xu, Duo[1,2];Guo, Dan[1,2];Han, Huan-Xing[3];Li, Da-Wei[1,2];Ma, Wei[1,2]

机构：[1]East China Univ Sci & Technol, Key Lab Adv Mat, Shanghai 200237, Peoples R China;[2]East China Univ Sci & Technol, Sch Chem & Mol Engn, Shanghai 200237, Peoples R China;[3]Aliex Technol Grp Co Ltd, 152,Lane 468,North Hengshahe Rd, Shanghai 201108, Peoples R China

年份：2020

卷号：56

期号：19

起止页码：2933

外文期刊名：CHEMICAL COMMUNICATIONS

收录：;EI(收录号：20201208312060);WOS:【SCI-EXPANDED(收录号:WOS:000519216400023)】；

基金：We acknowledge the financial support from the Shanghai Pujiang Program (2019PJD010), the National Natural Science Foundation of China (21775043), Shanghai Municipal Natural Science Fund (19ZR1472100), Shanghai Sailing Program (18YF1405700), Shanghai Strategic Emerging Industry Major Project (AI/RD2017-03) and Xiamen University Opening Project of PCOSS (201901).

语种：英文

外文关键词：Immunology - Biomarkers - Silver nanoparticles

摘要：We developed a novel enzyme-free amplified SERS immunoassay by combining silver nanoparticle (AgNP)-linked immunoreaction and SERS transduction for the detection of disease biomarkers. As a proof of concept, our method was successfully illustrated with the disease biomarker alpha-fetoprotein with the detection limit of 3.3 x 10(-13) g mL(-1) and a double-blind experiment consisting of tens of serum samples was performed to confirm its reliability.