文献类型：期刊文献

英文题名：Preparation and evaluation of a polymer-based sulfobetaine zwitterionic stationary phase

作者：Liu, Xuefang[1];Jiang, Yu[1];Zhang, Feifang[1];Li, Zongying[1];Yang, Bingcheng[1]

机构：[1]East China Univ Sci & Technol, Engn Res Ctr Pharmaceut Proc Chem, Sch Pharm, Minist Educ, 130 Meilong RD, Shanghai 200237, Peoples R China

年份：2021

卷号：1649

外文期刊名：JOURNAL OF CHROMATOGRAPHY A

收录：;EI(收录号：20212410514038);WOS:【SCI-EXPANDED(收录号:WOS:000653682500019)】；

基金：This work was sponsored by National Natural Science Foundation of China (No. 21974044) and National Key Research and Development Program of China (2018YFC1603500).

语种：英文

外文关键词：Hydrophilic interaction liquid chromatography; Zwitterionic stationary phase; Polymer; Saccharide separation; Column bleeding

摘要：We describe a polymer-based sulfobetaine zwitterionic stationary phase for hydrophilic interaction liquid chromatography (HILIC). It was prepared by grafting acrylamide-type sulfobetaine monomer instead of common methacrylate-type sulfobetaine onto hydrolysed poly (glycidyl methacrylate-divinylbenzene) (GMA-DVB) microspheres via pendant double bonds of DVB. The phase has been characterized by elemental analysis, scanning electron micrograph and N-2 adsorption-desorption experiment. It shows wider pH tolerance range (from 2 to 12) and excellent separation ability towards common strong polar analytes such as nucleosides and nucleic bases, water-soluble vitamins, amino acids, inorganic anions and cations. Notably, it exhibits negligible baseline noise level (similar to 0.15 pA) under typical HILIC mobile phase. Excellent selectivity in separation of alpha- and beta-anomers of reducing sugars and lactose/lactulose has also been observed. (c) 2021 Elsevier B.V. All rights reserved.