文献类型：期刊文献

英文题名：y The regulatory role of sulfated polysaccharides in facilitating rhBMP-2-induced osteogenesis

作者：Chen, Han[1,2];Yu, Yuanman[1,2];Wang, Chenmin[1,2];Wang, Jing[1,2];Liu, Changsheng[2,3]

机构：[1]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[2]East China Univ Sci & Technol, Minist Educ, Engn Res Ctr Biomed Mat, Shanghai 200237, Peoples R China;[3]East China Univ Sci & Technol, Minist Educ, Key Lab Ultrafine Mat, Shanghai 200237, Peoples R China

年份：2019

卷号：7

期号：10

起止页码：4375

外文期刊名：BIOMATERIALS SCIENCE

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000487753500032)】；

基金：The authors wish to express their gratitude to the financial supports from the National Natural Science Foundation of China (No. 31330028, and 31870953), the National Natural Science Foundation of China for Innovative Research Groups (No. 51621002), and the International Cooperation Project and Research Project of Shanghai Science and Technique Committee (15DZ1942502). This study was also supported by the 111 Project (B14018).

语种：英文

摘要：Sulfated polysaccharides have received much attention in recent years due to their special biological activities, especially the regulation of the biological activity of growth factors such as the representative inductive growth factor recombinant human bone morphogenetic protein-2 (rhBMP-2). However, the regulatory mechanisms from the aspect of the molecular chain structure have rarely been reported. In this article, we selected three kinds of sulfonates containing different backbone structures and functional groups, 2-N,6-O-sulfated chitosan (26 SCS), sulfated dextran (DSS) and poly(sodium-p-styrenesulfonate) (PSS), to explore the interaction between them and rhBMP-2. From in vivo and in vitro osteogenesis-related experiments, 26 SCS showed the best promoting effect on rhBMP-2 induced osteogenic differentiation and the sulfated amino group in 26 SCS could specifically bind to rhBMP-2. These findings indicated that the polysaccharide chain structure was a prerequisite for the synergy effect between 26 SCS and rhBMP-2; the effective combination of -SO3- and rhBMP-2 was an important factor in protecting the bioactivity of rhBMP-2. In addition, the presence of the sulfated amino group was the key factor in the specific binding between 26 SCS and rhBMP-2 and provided the possibility of capturing factors in vivo.