文献类型：期刊文献

英文题名：Trans-generational effect of neurotoxicity and related stress response in Caenorhabditis elegans exposed to tetrabromobisphenol A

作者：Liu, Fuwen[1];Luo, Qishi[2];Zhang, Ying[1];Huang, Kai[1];Cao, Xue[1];Cui, Changzheng[1,2];Lin, Kuangfei[1];Zhang, Meng[1]

机构：[1]East China Univ Sci & Technol, Sch Resource & Environm Engn, State Environm Protect Key Lab Environm Risk Asse, Shanghai 200237, Peoples R China;[2]Yonker Environm Protect Co Ltd, Branch Shanghai, Shanghai 200051, Peoples R China

年份：2020

卷号：703

外文期刊名：SCIENCE OF THE TOTAL ENVIRONMENT

收录：;EI(收录号：20194807737482);WOS:【SCI-EXPANDED(收录号:WOS:000505924300191)】；

基金：The authors have no conflicts of interest. The C. elegans including Wild-type and transgenic strains were supplied by the Caenorhabditis Genetics Center. This research was supported by the National Key Research and Development Program of China (No. 2018YFC1803304 and No. 2018YFC1800705), National Natural Science Foundation of China (No. 51708223).

语种：英文

外文关键词：TBBPA; Caenorhabditis elegans; Trans-generational effect; Neurotoxicity; Environmental risk

摘要：Tetrabromobisphenol A (TBBPA), one of the most common brominated flame retardants, has been associated with immunotoxicity, neurotoxicity, and reproductive toxicity. However, little attention has been focused on understanding the trans-generational effects of TBBPA. The present study used the Caenorhabditis elegans (C. elegans) animal model to evaluate the trans-generational effects of neurotoxicity induced by environmentally relevant concentrations of TBBPA (0, 0.1, 1, 10, 100, and 1000 mu g/L). Multiple indicators including physiological effects (body length, brood size, head thrashes, body bends, and crawling trajectory), degree of neuronal damage (dopamine, GABAergic, and glutamatergic neurons), oxidative stress-related biochemical indicators (superoxide dismutase [SOD] activity, catalase [CAT] enzyme, malondialdehyde [MDA] production, and reactive oxygen species [ROS] accumulation), and stress-related gene expressions have been evaluated in the exposed parental C. elegans generation (G(1)) and their progeny (G(2)) under TBBPA-free conditions. The results showed that TBBPA exposure induced adverse effects on physiological endpoints, among which body bends and head thrashes were the most sensitive ones, detected above 1 mu g/L in G(1) and 100 mu g/L in G(2) nematodes, respectively. After contaminant exposure, the three neurons revealed damage related to neurobehavioral endpoints, with no hereditary effects in the progeny. The oxidative stress-related biochemical endpoints demonstrated that when the exposure concentrations were above 1 mu g/L in maternal worms, impairment can be detected in both generations, but the progeny recovered at low toxicity concentration (1-100 mu g/L). The integrated target gene expression profiles were clearly altered in G(1) and G(2) worms at concentrations between 1 and 1000 mu g/L, and a more significant difference existed in two generations of nematodes at low levels (1-10 mu g/L) of TBBPA. Studing trans-generational neurotoxicity and the underlying mechanism can generate a precise evaluation of the environmental risk of TBBPA. (C) 2019 Elsevier B.V. All rights reserved.