文献类型：期刊文献

英文题名：Multi-component HPLC fingerprinting of radix salviae miltiorrhizae and its LC-MS-MS identification

作者：Hu, P; Liang, QL; Luo, GA; Zhao, ZZ; Jiang, ZH

机构：[1]E China Univ Sci & Technol, Modern Engn Ctr Trad Chinese Med, Sch Chem & Pharmaceut, Shanghai 200237, Peoples R China;[2]Hong Kong Baptist Univ, Sch Chinese Med, Kowloon, Hong Kong, Peoples R China;[3]Tsing Hua Univ, Anal Ctr, Beijing 100084, Peoples R China

年份：2005

卷号：53

期号：6

起止页码：677

外文期刊名：CHEMICAL & PHARMACEUTICAL BULLETIN

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000229861400018)】；

语种：英文

外文关键词：Salvia miltiorrhiza; HPLC fingerprint; LC-MS-MS; salvianolic acid; tanshinone

摘要：The multi-component fingerprinting method of Radix Salviae Miltiorrhizae (Root of Salvia miltiorrhiza B-GE.), an important and popular medicinal herb in traditional Chinese medicine, was studied using reverse-phase HPLC and LC-MS-MS. Extract containing both the water-soluble phenolic compounds and nonpolar diterpenoid compounds known to be the herb's main bioactive components was prepared by a two-step extractive procedure. An HPLC fingerprinting method which can simultaneously separate these two types of compounds was established with gradient elution mode and photodiode array detection at 280 nm. Eighteen peaks in this HPLC fingerprint were structurally identified by employing LC-MS-MS techniques. The electrospray ionization (ESI) MS-MS spectra of most salvianolic acids displayed a characteristic behavior of loss of danshensu and caffeic acid moieties, while those of tanshinones showed a particular behavior of loss of H2O which is quite different from the fragmentation pattern in electron ionization mass spectrometry (EI-MS). The HPLC fingerprints of 7 batches of crude drugs showed similar separation pattern and provided much chemical information of the pharmacologically-active compounds in the crude drugs, which is useful for the authentication and quality evaluation of this medicinal herb.