文献类型：期刊文献

英文题名：FRET-Based Mito-Specific Fluorescent Probe for Ratiometric Detection and Imaging of Endogenous Peroxynitrite: Dyad of Cy3 and Cy5

作者：Jia, Xiaotong[1];Chen, Qiangqiang[2];Yang, Yingfang[2];Tang, Yao[1];Wang, Rui[2];Xu, Yufang[1];Zhu, Weiping[1];Qian, Xuhong[1]

机构：[1]East China Univ Sci & Technol, Sch Pharm, Shanghai Key Lab Chem Biol, State Key Lab Bioreactor Engn, Meilong Rd 130, Shanghai 200237, Peoples R China;[2]East China Univ Sci & Technol, Sch Pharm, Shanghai Key Lab New Drug Design, Meilong Rd 130, Shanghai 200237, Peoples R China

年份：2016

卷号：138

期号：34

起止页码：10778

外文期刊名：JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

收录：;EI(收录号：20163602780018);WOS:【SCI-EXPANDED(收录号:WOS:000382513300016)】；

基金：This work was supported by the financial support from National Natural Science Foundation of China (21236002, 21476077, and 21302125), and the Fundamental Research Funds for the Central Universities. We thank Prof. Youjun Yang for insightful discussions and help with manuscript preparation.

语种：英文

外文关键词：Fluorescence spectroscopy - Probes

摘要：Peroxynitrite (OONO-) is profoundly implicated in health and disease. The physiological and pathological outcome of OONO- is related to its local Concentration, and hence, a reliable OONO- assay is highly desired. We have developed a FRET-based small molecule fluorescent probe (PNCy3Cy5), harnessing the differential reactivity of Cy3 and Cy5 toward OONO- by fine-tuning. It exhibits high detection sensitivity and yields a ratiometric fluorescent signal. We have exemplified that it can be applied in semiquantitative determination of OONO- in living cells. Notably, it specifically localizes in mitochondria where endogenous OONO- is predominantly generated. Thus, PNCy3Cy5 is a promising molecular tool for peroxynitrite biology.