文献类型：期刊文献

英文题名：Enhanced liquid-liquid phase separation of stress granules in a reconstructed model and their cytoplasmic targeting using a DNA nanodevice

作者：Liao, Yue[1];Fan, Chunyu[1];Zheng, Jiaxin[1];Liu, Caixia[1];Zhu, Weiping[1,3];Xu, Yufang[1];Qian, Xuhong[1,2];Yang, Yangyang[1]

机构：[1]East China Univ Sci & Technol, Sch Pharm, Shanghai Key Lab Chem Biol, Shanghai 200237, Peoples R China;[2]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[3]East China Univ Sci & Technol, Shanghai Frontiers Sci Ctr Optogenet Tech Cell Met, Sch Pharm, Shanghai 200237, Peoples R China

年份：2025

卷号：13

期号：5

起止页码：1744

外文期刊名：JOURNAL OF MATERIALS CHEMISTRY B

收录：;WOS:【SCI-EXPANDED(收录号:WOS:001380478100001)】；

基金：This work was supported by the National Key Research and Development Programs (2022YFD1700400 and 2023YFD1700300) and the Shanghai Frontiers Science Center of Optogenetic Techniques for Cell Metabolism.

语种：英文

摘要：Biomolecular condensates (BCs) are crucial membraneless organelles formed through the process of liquid-liquid phase separation (LLPS) involving proteins and nucleic acids. These LLPS processes are tightly linked with essential cellular activities. Stress granules (SGs), functioning as cytoplasmic BCs, play indispensable roles in maintaining cellular homeostasis and are implicated in diseases like cancers and neurodegenerative disorders. However, devices that can regulate SG LLPS are lacking. Herein, a triangular prism-shaped DNA nanostructure containing polythymidine (Delta DNA(polyT)) is presented as a nanodevice to investigate the LLPS process of in vitro reconstructed SGs (rSGs), a mixture of marker protein G3BP1 and total RNAs. Our observations reveal that the concentration threshold required for rSG LLPS decreases upon addition of Delta DNA(polyT), suggesting an enhancement in SG LLPS efficiency. It is speculated that Delta DNA(polyT) can concentrate mRNAs onto its surface via polyT hybridization with poly-adenosine sequences (polyA) in mRNAs. This alteration in the spatial distribution of mRNAs subsequently affects the multivalency interactions between G3BP1 and mRNAs. Furthermore, Delta DNA(polyT) exhibits excellent colocalization with cytoplasmic SGs under stressed conditions. This DNA-based nanodevice presents a new artificial approach for the targeted regulation of BC LLPS and holds promise for future studies focusing on BCs.