文献类型：期刊文献

英文题名：A low-cost and simple paper-based microfluidic device for simultaneous multiplex determination of different types of chemical contaminants in food

作者：Zhang, Yali[1];Zuo, Peng[1];Ye, Bang-Ce[1]

机构：[1]E China Univ Sci & Technol, State Key Lab Bioreactor Engn, Lab Biosyst & Microanal, Shanghai 200237, Peoples R China

年份：2015

卷号：68

起止页码：14

外文期刊名：BIOSENSORS & BIOELECTRONICS

收录：;EI(收录号：20150200405094);WOS:【SCI-EXPANDED(收录号:WOS:000351248300003)】；

基金：This work was financially supported by National Natural Science Foundation of China (31101285, 21335003), the Key Grant Project of Chinese Ministry of Education (Grant 313019), the Shanghai Fund (13142200500), the Fundamental Research Funds for the Central Universities, and Open Funding Project of State Key Laboratory of Dairy Biotechnology (SKLDB).

语种：英文

外文关键词：Paper-based microfluidic; Multiplex detection; Heavy metal ions detection; Drug residue detection

摘要：It is difficult to carry out multiple detection of different type of chemicals because of the different chemical microenvironment requirements. Herein, a low-cost and simple paper-based microfluidic device integrated with fluorescence labeled single-stranded DNA (ssDNA) functionalized graphene oxide sensor was developed for the multiplex determination of different types of chemical contaminants in food. In this work, Cy5 labeled corresponding functional ssDNA for different analytes associated with graphene oxide (ssDNA-GO) were employed as core detection sensors to sensitively report the presence of the different type of chemicals as well as enlarge the chemical compatibility, which made it possible to simultaneous detect multiple chemicals under a same chemical microenvironment Paper microfluidic device can be easily fabricated and paper substrate also facilitated the integration of ssDNA-GO sensors via physical absorption. This device has been successfully applied in multiplex detection of heavy metal mercury (II) ion (Hg2+) and silver (I) ion (Ag+) and aminoglycoside antibiotics residues in food. It also provided enormous potential for applications of environmental monitoring and clinical diagnosis. (C) 2014 Elsevier B.V. All rights reserved.