文献类型：期刊文献

英文题名：Development of a fed-batch process for the production of anticancer drug TAT_m-survivin(T34A) in Escherichia coli

作者：Zhang, Haiyi[1];Zheng, Yu[1];Liu, Qinghai[1];Tao, Xinyi[1];Zheng, Wenyun[1];Ma, Xingyuan[1];Wei, Dongzhi[1]

机构：[1]E China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China

年份：2009

卷号：43

期号：2

起止页码：163

外文期刊名：BIOCHEMICAL ENGINEERING JOURNAL

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000263018000008)】；

基金：This work was Supported by a grant from the Scientific Researches to Tackle Key Problems of Shanghai (no. 054319928) and a foundation for Young teacher from Education Ministry of China (no. 20070251011).

语种：英文

外文关键词：TAT(m)-survivin(T34A); Escherichia coli; Fed-batch culture; Optimization; Acetic acid; Bioprocess design

摘要：A fed-batch process was developed for intracellular production of recombinant TAT(m)-survivin(T34A) in Escherichia coli under the control of T7 promoter. The effects of induction mode and nutritional conditions were investigated. Compared to the one-point addition of inducer, the step-wise addition of isopropyl beta-D-thiogalactopyranoside (IPTG) maintained higher plasmid stability and increased the production level by 52%. Insufficient glucose supply after induction was observed to control acetate accumulation effectively and improved the expression of the target gene evidently. Remarkably, the pre-induction supplement of inorganic nitrogen source had a positive influence on the production of TAT(m)-survivin(T34A). High ammonium concentration of 4.8 g l(-1) was the most efficient in enhancing the production level (as the percentage of total cellular protein) and the specific productivity of TAT(m)-survivin(T34A). As a result, the production of TAT(m)-survivin(T34A) was optimized from 11.6% to 36.8% of total cellular protein (corresponding to 1.68 gl((1)). The findings provide valuable information for optimization of recombinant protein expression. (C) 2008 Elsevier B.V. All rights reserved.