文献类型：期刊文献

英文题名：Ultrasmall Pt Nanozymes Immobilized on Spherical Polyelectrolyte Brushes with Robust Peroxidase-like Activity for Highly Sensitive Detection of Cysteine

作者：Sun, Liang[1];Fu, Zhinan[2];Ma, Enguang[1];Guo, Jiangtao[2];Zhang, Ziyu[2];Li, Wenxin[1];Li, Li[2];Liu, Zhiyong[1];Guo, Xuhong[1,2]

机构：[1]Shihezi Univ, Sch Chem & Chem Engn, Shihezi 832003, Peoples R China;[2]East China Univ Sci & Technol, State Key Lab Chem Engn, Shanghai 200237, Peoples R China

年份：2022

卷号：38

期号：42

起止页码：12915

外文期刊名：LANGMUIR

收录：;EI(收录号：20224313006865);WOS:【SCI-EXPANDED(收录号:WOS:000870992500001)】；

基金：? ACKNOWLEDGMENTS The authors appreciate the financial support from the Key Scientific and Technological Project of Xinjiang Bingtuan (2018AB025) and the National Natural Science Foundation of China Grants (51773061 and 51761135128) . We thank the staff at beamline BL19U2 of the National Facility for Protein Science in Shanghai (NFPS) at SSRF for assistance with SAXS during data collection.

语种：英文

外文关键词：Amino acids - Catalyst activity - Catalytic oxidation - Color - Colorimetry - Free energy - Polyelectrolytes

摘要：Distinct platinum (Pt) nanozymes as peroxidase mimics have received extensive interest owing to their outstanding catalytic activity, high environmental tolerance, lower consumption, and great potential in replacing natural enzymes. However, easy agglomeration of Pt nanoparticles (Pt NPs) resulting from the high surface free energy significantly decrease their peroxidase-like activity. Herein, spherical polyelectrolyte brush (SPB)-stabilized ultrasmall Pt NPs (SPB@Pt NPs) were prepared by a novel synthetic strategy where the SPB not only performed as a nanoreactor for the synthesis of ultrasmall Pt NPs but also greatly stabilized Pt NPs against aggregation. The well-defined SPB@Pt NP nanozymes exhibited outstanding peroxidase-like activity for the catalytic oxidation of colorless 3,3 ',5,5 '-tetramethylbenzidine (TMB) to blue oxidized TMB and were then used to establish a colorimetric sensor for rapid detection of cysteine, giving a limit of detection of 0.11 mu M. Moreover, the colorimetric detection system was demonstrated with outstanding performance in sensitive and selective detection of cysteine in the presence of several interference molecules. From these results, SPB@Pt NPs have been regarded as promising peroxidase mimics for a large number of applications such as in biosensing, biomedicine, the food industry, and environmental chemistry.